timsTOF SCP

timsTOF SCP

Expanding the horizons of single cell research

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timsTOF SCP

Benefits of timsTOF SCP

Unbiased proteomics
with good reproducibility, robustness and coverage of about 1500 proteins per cell
Signaling pathway studies
timsTOF SCP has the sensitivity to revolutionize the use of phosphoproteomics for the study of signaling pathways in cancer.
Minimal sample load (<200 ng)
It allows analysis of hundreds of samples with minimal sample load (<200 ng) at sequencing speeds exceeding 100 Hz and with uncompromised proteomic depth.
Immunopeptidomics studies
timsTOF SCP is ideal for immunpeptidomics for neo-antigen discovery where the available material is limited, as in needle biopsies.

Applications

Shotgun proteomics
Shotgun proteomics
Single cell proteomics
Single cell proteomics
Ideal for immunopeptidomics
Ideal for immunopeptidomics
4D proteomics
4D proteomics
PTM analysis to complement scRNA-seq
PTM analysis to complement scRNA-seq
Epiproteomics
Epiproteomics
Tumor microenvironment
Tumor microenvironment

Assays/Consumables/Software

Glass capillaries
PHOX CROSSLINKER
FILTER SET CSI
Peaks Studio
MaxQuant

Features

Ultra-High Sensitivity

Ultra-High Sensitivity

Ground-breaking design of a novel ion source geometry for up to five times higher ion transfer and ultra-high robustness.

Robustness

Robustness

Double orthogonal reflection into trapped ion mobility spectrometry (TIMS) enables routine operation over thousands of samples without instrument cleaning.

Data Completeness

Data Completeness

Data independent acquisition–parallel accumulation serial fragmentation (dia-PASEF) pushes the limits of reproducible quantification to pave the way to study cell heterogeneity at scale.

Acquisition Speed

Acquisition Speed

High acquisition speed combined with dia-PASEF sensitivity enables analysis at short liquid chromatography (LC) runs without chromatographic dilution of very low sample amounts.

timsTOF SCP

timsTOF SCP

Expanding the horizons of single cell research

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Workflow of the System

timsTOF SCP - Expanding the Horizons of Single Cell Research

Related Videos

The timsTOF SCP features a modified ion source geometry that includes 1 mm capillary identification for five times higher ion transfer into an additional higher pressure stage ion funnel and 8-stage differentially pumped vacuum system. The larger capillary yields ultra-high sensitivity and the additional orthogonal ion reflection and subsequent funnel offers a separate, differentially pumped stage, maintaining the system robustness expected from the timsTOF instrument series.
timsTOF SCP results in an almost 5x improvement in ion current. When combined with the Evosep One Whisper method running at 100 nL/min flow rate, and the dia-PASEF method, sensitivity gains of about 100X over previous high-flow results with the Evosep One are achieved. This enables unbiased proteomics at the true single cell level with good reproducibility, robustness and coverage of about 1500 proteins per cell for the first time.

The highly unique TIMS design enables unmatched duty cycle (up to 100%), significant improvement in IMS resolution (R>200), unprecedented collisional cross section (CCS) reproducibility and high sensitivity.
timsTOF combines high ion mobility resolution with ultra-high resolution QTOF technology for optimal performance. imeXTM technology further boosts the functionality by making ion mobility resolution an adjustable parameter.
Investigating samples in imeX survey mode can lead to mobility-resolved full scans, and then extending workflow with a detailed examination of selected compounds and precise collisional cross sections (CCS). Increased ion mobility resolution up to 200 for unique insights into samples can be acheived in imeX ultra mode.

Data-independent acquisition dia-PASEF is both more sensitive and selective than traditional DIA approaches as it applies the PASEF principle to combine the advantages of DIA with the inherent ion efficiency of PASEF. Over the entire liquid chromatography-mass spectrometry (LC-TIMS-MS)/MS dia-PASEF run, a perfect data cuboid is created containing m/z, ion mobility (CCS), retention time and intensity. TIMS separation increases selectivity, excludes singly charged precursors from fragmentation and cleans up the sample by concentrating signals  from noise. Making use of the correlation of molecular weight and CCS coded information from the dual-TIMS funnel, dia-PASEF enables highly confident identification.

The timsTOF fleX combines 4D-Omics with MALDI Imaging technology, including smartbeam 3D laser optics to be a fast measurement all in one platform. 
It is a dual source instrument ideal for SpatialOMx®. It conducts robust ESI measurements and spatially resolves a wide range of molecules directly from tissue using one platform. 
During operation, software activation of the smartbeam 3D laser is the only change in the source region to change from ESI to MALDI in seconds.

timsTOF Flex MALDI 2 provides post ionization technique which reduces ion suppression effects and improves sensitivity by orders of magnitude. After the initial MALDI process, a second laser, sitting parallel to the sample surface, fires into the evolving plume and post-ionizes neutral (mainly matrix) molecules. A charge transfer from post-ionized matrix molecules to neutral analyte molecules leads to an amazing sensitivity gain for many analytes.

MALDI-2 post-ionization compensates the lower sample amount per pixel, making even the finest molecular distributions visible. Combining MALDI-2 post-ionization with microGRID enabled high spatial resolution opens the field for the analysis of the smallest unit of eukaryotic life - single cells.

TimsTOF MALDI Pharmapulse (MPP) uses a dual MALDI / ESI ion source with industry-leading 10 kHz smartbeam™ 3D laser which enable uHTS compatible speed and throughput. It supports rapid gas-phase separation of isobars, and even isomers, by exploiting the molecular collisional cross-section. This, in combination with routine 50,000 mass resolution in QTOF-MS detection facilitates revolutionary levels of assay specificity. It features the extreme speed and proven robustness of MALDI in the essence of tims technology.

It has an automation interface which supports HTS application of drug discovery. It has MPP 2023 which enables seamless transfer of data and results to downstream analysis software.

Faster separation of isobars and isomers
It is capable of separating isobars and even isomers on a timescale of ≤ 1 sec per separation cycle.

Data quality delivered at uncompromised HTS speed
Readout of a 1536 formatted sample plate in high-resolution MS or MS/MS mode takes less than 10 minutes

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