Benefits of timsTOF Flex - MALDI 2
Reduced ion suppression effects and improved sensitivity
Study involving small molecules desorbed from tissues sections such as lipidomics and metabolomics involving biomolecules from a wide variety of chemical classes
High spatial resolution up to 5 µm results in a drastically decreased ablated and ionized sample material
It translates to more than double the number of molecules detected per pixel resulting in much improved physiological context
Targeted organ study
Study of endogenous molecules
Metabolomics
Study of xenobiotics
Lipidomics
Tissue imaging
Applications
Features
Sensitivity
MALDI-2 increases sensitivity by up to 1-3 orders of magnitude depending on sample, analyte and matrix.
Dimensionality
MALDI-2 ionizes a wider range of chemical classes by reducing ion suppression effects, expanding chemical range for targeted analytes in MALDI Imaging.
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Workflow of the System
MALDI-2 Technology
Related Videos
timsTOF Flex MALDI 2 provides post ionization technique which reduces ion suppression effects and improves sensitivity by orders of magnitude. After the initial MALDI process, a second laser, sitting parallel to the sample surface, fires into the evolving plume and post-ionizes neutral (mainly matrix) molecules. A charge transfer from post-ionized matrix molecules to neutral analyte molecules leads to an amazing sensitivity gain for many analytes.
MALDI-2 post-ionization compensates the lower sample amount per pixel, making even the finest molecular distributions visible. Combining MALDI-2 post-ionization with microGRID enabled high spatial resolution opens the field for the analysis of the smallest unit of eukaryotic life - single cells.
The highly unique TIMS design enables unmatched duty cycle (up to 100%), significant improvement in IMS resolution (R>200), unprecedented collisional cross section (CCS) reproducibility and high sensitivity.
timsTOF combines high ion mobility resolution with ultra-high resolution QTOF technology for optimal performance. imeXTM technology further boosts the functionality by making ion mobility resolution an adjustable parameter.
Investigating samples in imeX survey mode can lead to mobility-resolved full scans, and then extending workflow with a detailed examination of selected compounds and precise collisional cross sections (CCS). Increased ion mobility resolution up to 200 for unique insights into samples can be acheived in imeX ultra mode.
Data-independent acquisition dia-PASEF is both more sensitive and selective than traditional DIA approaches as it applies the PASEF principle to combine the advantages of DIA with the inherent ion efficiency of PASEF. Over the entire liquid chromatography-mass spectrometry (LC-TIMS-MS)/MS dia-PASEF run, a perfect data cuboid is created containing m/z, ion mobility (CCS), retention time and intensity. TIMS separation increases selectivity, excludes singly charged precursors from fragmentation and cleans up the sample by concentrating signals from noise. Making use of the correlation of molecular weight and CCS coded information from the dual-TIMS funnel, dia-PASEF enables highly confident identification.
The timsTOF fleX combines 4D-Omics with MALDI Imaging technology, including smartbeam 3D laser optics to be a fast measurement all in one platform.
It is a dual source instrument ideal for SpatialOMx®. It conducts robust ESI measurements and spatially resolves a wide range of molecules directly from tissue using one platform.
During operation, software activation of the smartbeam 3D laser is the only change in the source region to change from ESI to MALDI in seconds.
timsTOF Flex MALDI 2 provides post ionization technique which reduces ion suppression effects and improves sensitivity by orders of magnitude. After the initial MALDI process, a second laser, sitting parallel to the sample surface, fires into the evolving plume and post-ionizes neutral (mainly matrix) molecules. A charge transfer from post-ionized matrix molecules to neutral analyte molecules leads to an amazing sensitivity gain for many analytes.
MALDI-2 post-ionization compensates the lower sample amount per pixel, making even the finest molecular distributions visible. Combining MALDI-2