Benefits of LabChip® GXII Touch™ Protein Characterization System
Adeno-associated virus (AAV) particles are one of the primary gene delivery vehicles used in clinical research because they facilitate long-term transgene expression in animal models with little associated toxicity and have low immunogenicity in humans. These particles could potentially be used to deliver protein receptors or nucleic acids to treat diseases caused by mutant genes. To study AAV particles, researchers often use SDS-PAGE with silver stain, a time and labor-intensive process which generally has low reproducibility and only yields qualitative data. The Labchip® GX Touch™ protein characterization system offers an automated alternative to gel electrophoresis increasing both throughput and data quality. This innovative instrument supports many assays for characterization of proteins and nucleic acids.
Sample preparation and characterization of proteins for quality by design (QbD) studies are important parts of process development; however, these studies, including design of experiments(DoE), can be time consuming and sample analysis can easily exceed the capacity of most labs. Protein characterization involves both upstream protein purification optimization, as well as the downstream characterization of its purity, structure and function. Miniaturization of small-scale protein purification provides greater process optimization with larger QbD and DOE studies allowing for parallel processing of samples for increased statistical significance and greater breadth of variables within experimental shorter time frames.
The LabChip® GXII Touch™ Protein Characterization system is ideal for SARS-CoV-2 protein subunit vaccine development. This system enables high-throughput protein sizing and quantification to support:
Recombinant subunit expression optimization and tracking
Subunit purification tracking
Formulation development- excipient assessment, purity, stability, modification, aggregation, and degradation
Product Quality Assessment- purity, stability, modification, aggregation, and degradation
Virus like particle (VLP) characterization
The ProteinEXact™ assay, combined with the LabChip® GX II Touch™ HT protein characterization system creates an efficient system to rigorously analyze asymmetrical bsAbs based on molecular weight sizing. The system can be used to study bsAbs: concentration, molecular weight, percent purity and charge variants.
Applications
Assays/Consumables/Software
Automated QC solution for Biotherapeutics
Protein Characterization
Proteins differ from each other in their size, molecular structure and physiochemical properties. These differences allow for protein analysis and characterization by separation and identification.
Flexible throughput
make it cost-efficient – 96-well or 384-well (HT) format
Quality Control
Rapid peak quantification and quality control throughout the protein purification workflow.
Data Visualization Options
E-gram, virtual gel, or data table format
Easy to use software
Track relevant user access and data history parameters with FDA 21 CFR Part 11 compatible software
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Workflow of the System
Characterize your proteins in under a minute
Related Videos
The LabChip® GXII Touch™ protein characterization system offers an automated alternative to traditional methods by streamlining slab gel electrophoresis, while also providing the increased throughput and data quality required by biotherapeutics and genomics workflows. This flexible instrument supports multiple assays for characterizing both proteins and nucleic acids.
The highly unique TIMS design enables unmatched duty cycle (up to 100%), significant improvement in IMS resolution (R>200), unprecedented collisional cross section (CCS) reproducibility and high sensitivity.
timsTOF combines high ion mobility resolution with ultra-high resolution QTOF technology for optimal performance. imeXTM technology further boosts the functionality by making ion mobility resolution an adjustable parameter.
Investigating samples in imeX survey mode can lead to mobility-resolved full scans, and then extending workflow with a detailed examination of selected compounds and precise collisional cross sections (CCS). Increased ion mobility resolution up to 200 for unique insights into samples can be acheived in imeX ultra mode.
Data-independent acquisition dia-PASEF is both more sensitive and selective than traditional DIA approaches as it applies the PASEF principle to combine the advantages of DIA with the inherent ion efficiency of PASEF. Over the entire liquid chromatography-mass spectrometry (LC-TIMS-MS)/MS dia-PASEF run, a perfect data cuboid is created containing m/z, ion mobility (CCS), retention time and intensity. TIMS separation increases selectivity, excludes singly charged precursors from fragmentation and cleans up the sample by concentrating signals from noise. Making use of the correlation of molecular weight and CCS coded information from the dual-TIMS funnel, dia-PASEF enables highly confident identification.
The timsTOF fleX combines 4D-Omics with MALDI Imaging technology, including smartbeam 3D laser optics to be a fast measurement all in one platform.
It is a dual source instrument ideal for SpatialOMx®. It conducts robust ESI measurements and spatially resolves a wide range of molecules directly from tissue using one platform.
During operation, software activation of the smartbeam 3D laser is the only change in the source region to change from ESI to MALDI in seconds.
timsTOF Flex MALDI 2 provides post ionization technique which reduces ion suppression effects and improves sensitivity by orders of magnitude. After the initial MALDI process, a second laser, sitting parallel to the sample surface, fires into the evolving plume and post-ionizes neutral (mainly matrix) molecules. A charge transfer from post-ionized matrix molecules to neutral analyte molecules leads to an amazing sensitivity gain for many analytes.
MALDI-2 post-ionization compensates the lower sample amount per pixel, making even the finest molecular distributions visible. Combining MALDI-2 post-ionization with microGRID enabled high spatial resolution opens the field for the analysis of the smallest unit of eukaryotic life - single cells.
TimsTOF MALDI Pharmapulse (MPP) uses a dual MALDI / ESI ion source with industry-leading 10 kHz smartbeam™ 3D laser which enable uHTS compatible speed and throughput. It supports rapid gas-phase separation of isobars, and even isomers, by exploiting the molecular collisional cross-section. This, in combination with routine 50,000 mass resolution in QTOF-MS detection facilitates revolutionary levels of assay specificity. It features the extreme speed and proven robustness of MALDI in the essence of tims technology.
It has an automation interface which supports HTS application of drug discovery. It has MPP 2023 which enables seamless transfer of data and results to downstream analysis software.
Faster separation of isobars and isomers
It is capable of separating isobars and even isomers on a timescale of ≤ 1 sec per separation cycle.
Data quality delivered at uncompromised HTS speed
Readout of a 1536 formatted sample plate in high-resolution MS or MS/MS mode takes less than 10 minutes
Related Resources
DART-OS enables a very simple workflow, for a wide range of sample types
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Start the system - switch on the source and start the VAPUR pump
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Spot or dose the sample onto an OpenSpot® sample card in the clearly marked area
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Slot the card into the system to guide your sample into exactly the right position for successful analysis
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Scan – the system will apply transmission mode DART passing a heated carrier gas through the sample
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Remove the card and discard