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QF PCR

QF PCR

A way of robust, reliable and accurate tests

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Expert Balance
Expert Balance

Expertly balances clinical utility and ease of use

Screen, identify & semi-quantify Known & Unknown targets with much more confidence

Identified Polar, a nonpolar compound in a single run

>12 min Run & Analysis time (Sample to Report)

Curated Library & Methods for over 6000+ Toxicology relevant compounds & their metabolites

 

New compound can be added in few clicks

UHPLC combined with true MSn and comprehensive Drug Libraries

Features of QF PCR

Single Report

Single Report

Single reports using GeneMarker™ and GeneMapper® software applications

Simple Analysis

Simple Analysis

Simple analysis and all validated kits.

Set

Set

Simple, Single tube reaction set.

Benefits of QF PCR

Easy determination of chromosome specific STR

The observation of an extra STR allele as either a three peak pattern in a 1:1:1 ratio or two peak pattern in a 2:1 or 1:2 peak ratio is diagnostic of the presence of an additional sequence, which in turn may represent an additional chromosome as in the case of a trisomy.

Least Turnaround Time

Turnaround reporting times of less than 24 hours from sample receipt are easily achievable

Best Compatibility with most systems

It is compatible with most commonly employed DNA extraction protocols, thermal cyclers and capillary electrophoresis instruments.

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An instrument for every need:

Integrated benchtop system for Research Labs

For Core Labs and High Sample Throughput

nCounter Life Science Assays:

nCounter Elements™:

Expandable with Additional Prep Station:

Enterprise Package:

PROSIGNA optional add-on:

Runs Per Day:

Throughput (Lanes Per Day):

Hands on Time:

Reaction Volume Required:

Linear Dynamic Range:

Throughput:

Yes

Yes

No

No

No

2

24

10 mins

Up to 35 µl

6 x10* total count

12 lanes/6 hours

Yes

Yes

Yes

Yes

No

4*

48-96*

15 mins

Up to 30 µl

7 x10* total count

12 lanes/2.5 hours

nCounter Sprint
nCounter Flex

Product Comparison

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UHPLC combined with true MSn and comprehensive drug libraries

Related Resources

Quantitative fluorescent polymerase chain reaction (QF-PCR) can be applied to the detection of chromosome copy number by amplification of repeat sequences at polymorphic loci. These repeat sequences are amplified by PCR, and the labelled products are separated by gel electrophoresis.

An allele pattern of two equal peaks within the same chromosomal region is diagnostic of two copies of the target region, whereas three peaks within the same chromosomal region or two peaks with a ratio of 2 : 1 are indicative of trisomy for the target region. This diagnostic approach was first suggested in 1993, and prospective studies were carried out in the mid 1990s, followed by the development of a QF-PCR-based test for sex chromosome imbalance.

QF PCR

QF PCR

A way of robust, reliable and accurate tests

Enquire Now
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