Cancer Liquid Biopsy – cfDNA Analysis
Circulating Tumor Cell Analysis at Single Cell Level
Liquid biopsy is a new diagnostic concept to investigate the molecular features of solid tumors by blood, saliva, urine, and any other body fluids which show a source of potential biomarkers.
In cancer patients, it is a simple and less invasive mean, representing a sustainable alternative to interrogate all tumor cells longitudinally, quantifying and characterizing the biological materials (DNAs, RNAs, proteins) which originate from cancer tissues.
Mutation detection in liquid biopsy, however, is challenged by the extremely low fraction of ctDNA in the cell-free DNA (cfDNA) samples, making accurate detection of rare mutations difficult.
Multiplex PCR-based NGS Target Enrichment Method For Precise Tumor DNA Analysis
Next-generation sequencing (NGS) has made it possible to detect a large number of mutations in many genes on lots of samples.
By using targeted NGS panels, sequencing can be focused on clinically relevant targets so that each target is sequenced thousands or tens of thousands of times to ensure a high degree of sensitivity.
High specificity is achieved using error-correction techniques to remove errors that arise from sequencing and amplification of the DNA.
Ultrafast, Confident Detection of Low-Frequency Variants
The CleanPlex® UMI Lung Cancer Panel is a targeted NGS assay designed for rapid and high confident detection of low-frequency variants across the hotspot regions of 23 genes associated with lung cancer.
The CleanPlex UMI Lung Cancer Panel offers a ultrafast and streamlined workflow. Starting from purified and quantitated DNA, the multiplex PCR-based protocol can be completed in just 3.5 hours, with 85 minutes of hands-on time, using a three-step, single-tube workflow. Each step consists of a thermal-cycling or incubation condition, followed by “with bead” purification using magnetic beads.
CleanPlex UMI Single-Tube Workflow
Confident Variant Detection with UMI Error Correction
CleanPlex UMI Technology features a simple and rapid workflow and uses proprietary molecular barcoding chemistry to uniquely label and differentiate the two strands of each DNA molecule.
The incorporated unique molecular identifiers (UMIs) allow PCR and sequencing errors to be corrected in the sequencing data, resulting in improved sensitivity and specificity for variant detection.