Detecting Low-Frequency Variants using a Targeted Amplicon Sequencing Approach And Molecular Barcodes

• The CleanPlex® UMI Lung Cancer Panel is a targeted resequencing assay designed for rapid and high confident detection of ultralow frequency variants across the hotspot regions of 23 genes associated with lung cancer.

• Based on CleanPlex UMI technology, this panel is specifically designed to work with cell-free DNA (cfDNA) and FFPE DNA for precise analysis of tumor DNA in liquid biopsy and tumor profiling applications.

• The panel is powered by Paragon Genomics’ CleanPlex UMI Technology which incorporates novel unique molecular identifiers (UMIs) to enable correction of PCR and sequencing errors.

• Starting with just 20 ng of DNA, sequencing-ready libraries can be prepared using a single-tube workflow in just 3.5 hours. Low-frequency variants below 0.5% allele frequency can be confidently detected with high specificity using just 30 ng of DNA.