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nCounter® PanCancer Progression Panel
Prosigna Score / ROR Score:
The ROR Score has been validated to predict the risk of recurrence of disease in ER+ breast cancer after surgery and treatment with 5 years of endocrine therapy. The ROR score depends upon the biology of the intrinsic subtypes, the proliferation score of the tumor, and the tumor size as shown in the equation below:
ROR = aRLumA + bRLumB + cRHer2 + dRBasal + eP + fT
Each of the R variables in the equation above indicate the Pearson correlation coefficient of the PAM50 expression profile for the tumor compared to each of the prototypical centroids for the intrinsic subtypes shown in the heatmap below. P is the proliferation score, which is the average gene expression profile of genes associated with cell-cycle progression and T is the tumor stage.
Figure: Heatmap of PAM50 genes by subtype. Red is higher expression and green is lower expression
Cancer progression involves multiple processes and mechanisms that are highly interconnected.
The nCounter PanCancer Progression Panel lets you perform multiplex gene expression analysis with 770 genes from each step in the cancer progression process including angiogenesis, extracellular matrix remodeling (ECM), epithelial-to-mesenchymal transition (EMT), and metastasis.
Angiogenesis: Angiogenesis is the biological process of generating new blood vessels from pre-existing vasculature and plays an important role in cancer progression as solid tumors require a blood supply to grow beyond few millimeters.
Extracellular matrix remodeling(ECM): ECM is an assembly of proteins and sugar that surrounds cells in solid tissue that provide mechanical structure to cells. The ECM must be remodeled in order to accommodate tumor growth. Several remodeling genes have further roles in progression, acting as transcription factors in metastatic growth.
Epithelial to mesenchymal transition (EMT): EMT is a process in which epithelial cells lose their polarity and cell to cell adhesion which is then undergoing biochemical changes that enable them to acquire mesenchymal-like properties. EMT is a process that cancer cells undergo which plays an important role in metastasis.
Metastasis: Metastasis is a collection of cellular processes commencing when cells migrate from the primary tumor to the bloodstream or lymph vessels to another part of the body and form new tumors. There are a set of metastasis suppressor genes that each potential new tumor site must avoid.
Quantify gene expression of metastatic growth and suppressor genes.
Rapidly and easily screen samples for biomarker discovery or drug mechanism of action to support your research.
3D-enabled for multi-analyte analysis with Vantage 3D™ Assays
GeoMx DSP COVID-19 Protein and RNA Analysis
Rapidly perform high-plex spatial analyses of the host response in FFPE or fresh frozen tissue using the GeoMx Digital Spatial Profiler (DSP). NanoString’s GeoMx DSP platform enables high-plex protein and RNA experiments in key areas of biology such as molecular response, cellular (immune) response, tissue damage, and drivers of individual susceptibility to severe forms of disease.
The GeoMx COVID-19 Immune Response Atlas, a ~1,850-plex RNA assay, enables spatial studies of the SARS-CoV-2 virus and host response. RNA targets include COVID-19 receptors and proteases, pulmonary alveolar type I and II markers, lung biology markers, viral response markers, and SARS-CoV-2 probes. RNA targets are profiled simultaneously using the GeoMx DSP and an Illumina next-generation sequencer (NGS) for readout. Users can run ACD RNAscope™ probes alongside GeoMx RNA probes to identify regions of interest.
A five-antibody custom, ready-to-go protein panel, with receptor, protease, and viral markers is available through the GeoMx Technology Access Program or for order through Abcam. This COVID-19 GeoMx-formatted Antibody is run with the 20-plex GeoMx Immune Cell Profiling Core (plus controls) with readout on the nCounter Analysis System. Users can add up to six 10-plex modules including the Immune Activation Status, Immune Cell Typing, and/or Cell Death modules to more deeply profile proteins involved in T cell activation and cell death. NanoString scientists can recommend commercially-available markers for lung epithelium, nasal epithelium, immune response markers, and the viral spike protein.
Comprehensive Gene Expression
Perform multiplex gene expression analysis with 770 genes from each step in the cancer progression process including: angiogenesis, extracellular matrix remodeling (ECM), epithelial-to-mesenchymal transition (EMT) and metastasis.
Flexible Sample Type
FFPE derived RNA, total RNA, fragmented RNA, cell lysates
Customizable with up to 55 additional user-defined genes with Panel Plus option
Investigate cancer progression and metastasis
The eight essential components of stem cell biology
Stem Cell Renewal:
Stem Cell Prolifiration
PSC Pluripotency Markers and Regulators
Naive State/Primed State
Histone Acetylation & Methylation
Oxidative Stress Response
Amino Acid Metabolism
Fatty Acid Metabolism
Differentiation Signaling and Pathways:
HOX Gene Activation
Endodermal /Ectodermal /Mesodermal Lineage Markers
Key Somatic Cell Types
Custom & Clinical Products
Daily Sample Throughput
Self-assembled, interchangeable probes, optimized for smaller validation projects with maximum flexibility
Self-assembled, interchangeable probes for high-throughput, sample multiplexing projects
User-designed, turn-key solution that comes ready-to-use
mRNA, CNV, Fusions
mRNA, miRNA, CNV, Fusions
Cell line drug screen, biomarker development, infectious disease
Gene Signature Development
Model Organism, Microbes, Agriculture
Please contact us for more info at email@example.com