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nCounter® Custom Solutions

nCounter® Custom Solutions

Prosigna Score / ROR Score:

The ROR Score has been validated to predict the risk of recurrence of disease in ER+ breast cancer after surgery and treatment with 5 years of endocrine therapy. The ROR score depends upon the biology of the intrinsic subtypes, the proliferation score of the tumor, and the tumor size as shown in the equation below:

ROR = aRLumA + bRLumB + cRHer2 + dRBasal + eP + fT

Each of the R variables in the equation above indicate the Pearson correlation coefficient of the PAM50 expression profile for the tumor compared to each of the prototypical centroids for the intrinsic subtypes shown in the heatmap below. P is the proliferation score, which is the average gene expression profile of genes associated with cell-cycle progression and T is the tumor stage.

Figure: Heatmap of PAM50 genes by subtype. Red is higher expression and green is lower expression

nCounter® Custom Solutions

nCounter® Gene Expression assays reduce time to data with a simple workflow of less than 15 minutes hands-on time and streamlined analysis, generating results in under 24 hours.  The platform is ideal for processing higher numbers of samples commonly seen in studies with plants, animals, and model organisms; minimal hands-on time maximizes productivity in the lab.

  • Profile up to 800 genes from a single sample

  • Flexible content allows you to select the organism/genes of your choice

  • Compatible with total RNA and crude lysate

  • No amplification, RT or library prep with as little as 25 ng input material

  • No technical replicates required due to direct, digital counting

  • Sample multiplexing with PlexSet™ reagents allows you to profile up to 96 genes in 96 samples with one run, generating up to 9,216 data points

  • Lysate-compatible protocol for processing multiple samples more efficiently

nCounter Workflow for Custom Gene Expression Assays

nCounter Workflow for Custom Gene Expression Assays

GeoMx DSP COVID-19 Protein and RNA Analysis

  • Rapidly perform high-plex spatial analyses of the host response in FFPE or fresh frozen tissue using the  GeoMx Digital Spatial Profiler (DSP). NanoString’s GeoMx DSP platform enables high-plex protein and RNA experiments in key areas of biology such as molecular response, cellular (immune) response, tissue damage, and drivers of individual susceptibility to severe forms of disease.

  • The GeoMx COVID-19 Immune Response Atlas, a ~1,850-plex RNA assay, enables spatial studies of the SARS-CoV-2 virus and host response. RNA targets include COVID-19 receptors and proteases, pulmonary alveolar type I and II markers, lung biology markers, viral response markers, and SARS-CoV-2 probes. RNA targets are profiled simultaneously using the GeoMx DSP and an Illumina next-generation sequencer (NGS) for readout. Users can run ACD RNAscope™ probes alongside GeoMx RNA probes to identify regions of interest.

  • A five-antibody custom, ready-to-go protein panel, with receptor, protease, and viral markers is available through the GeoMx Technology Access Program or for order through Abcam. This  COVID-19 GeoMx-formatted Antibody  is run with the 20-plex GeoMx Immune Cell Profiling Core (plus controls) with readout on the nCounter Analysis System. Users can add up to six 10-plex modules including the Immune Activation Status, Immune Cell Typing, and/or Cell Death modules to more deeply profile proteins involved in T cell activation and cell death. NanoString scientists can recommend commercially-available markers for lung epithelium, nasal epithelium, immune response markers, and the viral spike protein.

Custom CodeSets

Custom CodeSets – A turn-key solution that provides ready-to-use assay for up to 800 targets. Contact us to discuss your custom panel design with the bioinformatics team.

Elements™ TagSets

Elements TagSets– For small-scale projects, Elements chemistry enables the detection and quantitation of RNA and DNA targets by hybridizing to target-specific oligonucleotide probes, purchased from an oligo supplier. Contact us to discuss your design with the bioinformatics team.

PlexSet™ Reagents

PlexSet Reagents– Generate up to 9,216 data points per run; design custom probes or choose from pre-selected pathway panels. PlexSet chemistry enables the detection of RNA targets by hybridizing to target-specific oligonucleotide probes, purchased from an oligo supplier. Contact us to discuss your design with the bioinformatics team.

Plus Content

Rather have an off-the-shelf assay but with customization? Our Panel Plus option allows you to customize any off-the-shelf nCounter Gene Expression Panel by adding 6 to 55 user-defined probes. Visit the Panel Plus Page for more information. You may also use this option to update any Custom CodeSet previously ordered with new genes of your choice. Visit the Codeset Plus page for more information.

Design the content that is flexible regardless of the organism or gene. Choose from the given options:

The eight essential components of stem cell biology

Stemness
Pluripotency
Regulatory Signaling
Epigenetics
Mechano-Signaling
Metabolism
Differentiation Signaling
Lineage Specification

Stem Cell Renewal:

  • Stem Cell Prolifiration

  • Cell Cycle

  • Senescence/Quiescence

  • Autophagy

  • Apoptosis

  • Anti-Apoptosis

  • PSC Pluripotency Markers and Regulators

  • Naive State/Primed State

Regulatory Pathways:​

  • Wnt/B-catenin Pathway

  • Hedgehog Signaling

  • AP-1 Signaling

  • PI3K-AKTmTOR Pathway

  • MAPK Pathway

  • JAK/STAT Pathway

  • Notch Signaling

Epigenetic Mechanism:

  • DNA Methylation

  • Histone Acetylation & Methylation

Mechano-Signaling:

  • Rho/ROCK Signaling

  • integrin/Cadherin Signaling

  • Hippo Pathway

Metabolism:

  • Oxidative Stress Response

  • Hypoxia Response

  • Amino Acid Metabolism

  • Fatty Acid Metabolism

  • Glutamine Metabolism

  • Glucose Metabolism

Differentiation Signaling and Pathways:

  • TGFB Signaling

  • Cytoskeletal Reorganization

  • MET/EMT Signaling

  • HOX Gene Activation

Differentiation Lineage:

  • Endodermal /Ectodermal /Mesodermal Lineage Markers

  • Key Somatic Cell Types

Custom & Clinical Products

Assay Type
Product
Maximum Targets
Daily Sample Throughput
Analytes Offered
Common Applications

Custom codeSets

Elements TagSets

PlexSet Reagents

Self-assembled, interchangeable probes, optimized for smaller validation projects with maximum flexibility

Self-assembled, interchangeable probes for high-throughput, sample multiplexing projects

User-designed, turn-key solution that comes ready-to-use

216

96

800

24-96

24-96

192-1152

mRNA, CNV, Fusions

mRNA, Fusions

mRNA, miRNA, CNV, Fusions

Cell line drug screen, biomarker development, infectious disease

Gene Signature Development

Model Organism, Microbes, Agriculture

SKU
Description
Pack Size

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