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nCounter® PlexSet™ Reagents

nCounter® PlexSet™ Reagents

Prosigna Score / ROR Score:

The ROR Score has been validated to predict the risk of recurrence of disease in ER+ breast cancer after surgery and treatment with 5 years of endocrine therapy. The ROR score depends upon the biology of the intrinsic subtypes, the proliferation score of the tumor, and the tumor size as shown in the equation below:

ROR = aRLumA + bRLumB + cRHer2 + dRBasal + eP + fT

Each of the R variables in the equation above indicate the Pearson correlation coefficient of the PAM50 expression profile for the tumor compared to each of the prototypical centroids for the intrinsic subtypes shown in the heatmap below. P is the proliferation score, which is the average gene expression profile of genes associated with cell-cycle progression and T is the tumor stage.

Figure: Heatmap of PAM50 genes by subtype. Red is higher expression and green is lower expression

nCounter® PlexSet™ Reagents
  • Accelerate your cell line screening and high-throughput applications with nCounter PlexSetReagents for Gene Expression Analysis.

  • PlexSet technology enables multiplexed gene expression assays to be performed more efficiently and cost effectively for projects ranging up to 96 RNA targets.

  • PlexSetreagents enable researchers to reduce their hands-on time by eliminating the need for cDNA conversion, replicate utilization, or RNA purification.

GeoMx DSP COVID-19 Protein and RNA Analysis

  • Rapidly perform high-plex spatial analyses of the host response in FFPE or fresh frozen tissue using the  GeoMx Digital Spatial Profiler (DSP). NanoString’s GeoMx DSP platform enables high-plex protein and RNA experiments in key areas of biology such as molecular response, cellular (immune) response, tissue damage, and drivers of individual susceptibility to severe forms of disease.

  • The GeoMx COVID-19 Immune Response Atlas, a ~1,850-plex RNA assay, enables spatial studies of the SARS-CoV-2 virus and host response. RNA targets include COVID-19 receptors and proteases, pulmonary alveolar type I and II markers, lung biology markers, viral response markers, and SARS-CoV-2 probes. RNA targets are profiled simultaneously using the GeoMx DSP and an Illumina next-generation sequencer (NGS) for readout. Users can run ACD RNAscope™ probes alongside GeoMx RNA probes to identify regions of interest.

  • A five-antibody custom, ready-to-go protein panel, with receptor, protease, and viral markers is available through the GeoMx Technology Access Program or for order through Abcam. This  COVID-19 GeoMx-formatted Antibody  is run with the 20-plex GeoMx Immune Cell Profiling Core (plus controls) with readout on the nCounter Analysis System. Users can add up to six 10-plex modules including the Immune Activation Status, Immune Cell Typing, and/or Cell Death modules to more deeply profile proteins involved in T cell activation and cell death. NanoString scientists can recommend commercially-available markers for lung epithelium, nasal epithelium, immune response markers, and the viral spike protein.

Multiplex targets

Multiplex up to 96 custom probes across 96 samples to generate 9,216 data points per run.

Utilization of Lysed cells

Utilization of lysed cells eliminates the need for RNA purification and amplification; save resources with an efficient workflow that does not require cDNA conversions, replicates, or prior probe optimizations

Accurate reproducible results

Proven nCounter Digital Gene Expression technology provides excellent precision and reproducibility across a wide dynamic range

Rapid

Approximately 30 minutes of hands-on time and time to result is 24 hours only.

No upfront probe

No upfront probe optimization required

Minimum Input Material

50 ng of total purified RNA or 5,000 cells or 1 ng of RNA input with amplification

Provides a simple and robust method for multiplexing targets without the need to optimize probes or amplification conditions.

The eight essential components of stem cell biology

Stemness
Pluripotency
Regulatory Signaling
Epigenetics
Mechano-Signaling
Metabolism
Differentiation Signaling
Lineage Specification

Stem Cell Renewal:

  • Stem Cell Prolifiration

  • Cell Cycle

  • Senescence/Quiescence

  • Autophagy

  • Apoptosis

  • Anti-Apoptosis

  • PSC Pluripotency Markers and Regulators

  • Naive State/Primed State

Regulatory Pathways:​

  • Wnt/B-catenin Pathway

  • Hedgehog Signaling

  • AP-1 Signaling

  • PI3K-AKTmTOR Pathway

  • MAPK Pathway

  • JAK/STAT Pathway

  • Notch Signaling

Epigenetic Mechanism:

  • DNA Methylation

  • Histone Acetylation & Methylation

Mechano-Signaling:

  • Rho/ROCK Signaling

  • integrin/Cadherin Signaling

  • Hippo Pathway

Metabolism:

  • Oxidative Stress Response

  • Hypoxia Response

  • Amino Acid Metabolism

  • Fatty Acid Metabolism

  • Glutamine Metabolism

  • Glucose Metabolism

Differentiation Signaling and Pathways:

  • TGFB Signaling

  • Cytoskeletal Reorganization

  • MET/EMT Signaling

  • HOX Gene Activation

Differentiation Lineage:

  • Endodermal /Ectodermal /Mesodermal Lineage Markers

  • Key Somatic Cell Types

Custom & Clinical Products

Assay Type
Product
Maximum Targets
Daily Sample Throughput
Analytes Offered
Common Applications

Custom codeSets

Elements TagSets

PlexSet Reagents

Self-assembled, interchangeable probes, optimized for smaller validation projects with maximum flexibility

Self-assembled, interchangeable probes for high-throughput, sample multiplexing projects

User-designed, turn-key solution that comes ready-to-use

216

96

800

24-96

24-96

192-1152

mRNA, CNV, Fusions

mRNA, Fusions

mRNA, miRNA, CNV, Fusions

Cell line drug screen, biomarker development, infectious disease

Gene Signature Development

Model Organism, Microbes, Agriculture

nCounter PlexSet Titration Kit-96, Kit is set up to perform titration for 96-gene format prior to processing samples with PlexSet-96 reagents in 96 sample format. Only one kit is required for the entire project. CodeSet only—does not include Master Kits

Description

24 Assays

Pack Size

PS-GX-PTK-96 (CS0)

SKU

nCounter PlexSet Titration Kit-72, Kit is set up to perform titration for 72-gene format prior to processing samples with PlexSet-72 reagents in 96 sample format. Only one kit is required for the entire project. CodeSet only—does not include Master Kits.

Description

24 Assays

Pack Size

PS-GX-PTK-72 (CS0)

SKU

nCounter PlexSet Titration Kit-48, Kit is set up to perform titration for 48-gene format prior to processing samples with PlexSet-48 reagents in 96 sample format. Only one kit is required for the entire project. CodeSet only—does not include Master Kits.

Description

24 Assays

Pack Size

PS-GX-PTK-48 (CS0)

SKU

nCounter PlexSet Titration Kit-24, Kit is set up to perform titration for 24-gene format prior to processing samples with PlexSet-24 reagents in 96 sample format. Only one kit is required for the entire project. CodeSet only—does not include Master Kits.

Description

24 Assays

Pack Size

PS-GX-PTK-24 (CS0)

SKU

nCounter PlexSet Titration Kit-12, Kit is set up to perform titration for 12-gene format prior to processing samples with PlexSet-12 reagents in 96 sample format. Only one kit is required for the entire project. CodeSet only—does not include Master Kits

Description

24 Assays

Pack Size

PS-GX-PTK-12 (CS0)

SKU

nCounter PlexSet-96 Reagent Pack, For custom Gene Expression analysis utilizing 96 genes and 96 samples per nCounter run. Each pack of reagents is sufficient for 1 nCounter runs or 96 assays and requires 12 reaction Master kit. Tier discounting is available for purchase of 8 packs or more (768 samples or more). PlexSet purchases only— does not include Master Kits.

Description

96 Assays

Pack Size

PS-096-GX-96S (CS0)

SKU

nCounter PlexSet-72 Reagent Pack, For custom Gene Expression analysis utilizing 72 genes and 96 samples per nCounter run. Each pack of reagents is sufficient for 1 nCounter runs or 96 assays and requires 12 reaction Master kit. Tier discounting is available for purchase of 8 packs or more (768 samples or more). PlexSet purchases only— does not include Master Kits

Description

96 Assays

Pack Size

PS-072-GX-96S (CS0)

SKU

nCounter PlexSet-48 Reagent Pack, For custom Gene Expression analysis utilizing 48 genes and 96 samples per nCounter run. Each pack of reagents is sufficient for 1 nCounter runs or 96 assays and requires 12 reaction Master kit. Tier discounting is available for purchase of 8 packs or more (768 samples or more). PlexSet purchases only— does not include Master Kits.

Description

96 Assays

Pack Size

PS-048-GX-96S (CS0)

SKU

nCounter PlexSet-24 Reagent Pack, For custom Gene Expression analysis utilizing 24 genes and 96 samples per nCounter run. Each pack of reagents is sufficient for 2 nCounter runs or 192 assays and requires 24 reaction Master kit. Tier discounting is available for purchase of 4 packs or more (768 samples or more). PlexSet only—does not include Master Kits.

Description

192 Assays

Pack Size

PS-024-GX-96S (CS0)

SKU

nCounter PlexSet-12 Reagent Pack, For custom Gene Expression analysis utilizing 12 genes and 96 samples per nCounter run. Each pack of reagents is sufficient for 2 nCounter runs or 192 assays and requires 24 reaction Master kit. Tier discounting is available for purchase of 4 packs or more (768 samples or more). PlexSet purchases only—does not include Master Kits.

Description

192 Assays

Pack Size

PS-012-GX-96S (CS0)

SKU

nCounter PlexSet Titration Kit-96 Kit is set up to perform titration for 96-gene format prior to processing samples with PlexSet-96 reagents in 96 sample format. Only one kit is required for the entire project. CodeSet only—does not include Master Kits

Description

24 Assays

Pack Size

PS-GX-PTK-96
(CS0)

SKU

nCounter PlexSet-48 Reagent Pack For custom Gene Expression analysis utilizing 48 genes and 96 samples per nCounter run. Each pack of reagents is sufficient for 1 nCounter runs or 96 assays and requires 12 reaction Master kit. Tier discounting is available for purchase of 8 packs or more (768 samples or more). PlexSet purchases only— does not include Master Kits

Description

96 Assays

Pack Size

PS-048-GX-96S
(CS0)

SKU

nCounter PlexSet Titration Kit-48 Kit is set up to perform titration for 48-gene format prior to processing samples with PlexSet-48 reagents in 96 sample format. Only one kit i

Description

24 Assays

Pack Size

PS-GX-PTK-48
(CS0)

SKU

nCounter PlexSet-24 Reagent Pack For custom Gene Expression analysis utilizing 24 genes and 96 samples per nCounter run. Each pack of reagents is sufficient for 2 nCounter runs or 192 assays and requires 24 reaction Master kit. Tier discounting is available for purchase of 4 packs or more (768 samples or more). PlexSet only—does not include Master Kits

Description

192 Assays

Pack Size

PS-024-GX-192S
(CS0)

SKU

nCounter PlexSet-12 Reagent Pack For custom Gene Expression analysis utilizing 12 genes and 96 samples per nCounter run. Each pack of reagents is sufficient for 2 nCounter runs or 192 assays and requires 24 reaction Master kit. Tier discounting is available for purchase of 4 packs or more (768 samples or more). PlexSet purchases only—does not include Master Kits.

Description

192 Assays

Pack Size

PS-012-GX-192S
(CS0)

SKU

nCounter PlexSet-96 Reagent Pack For custom Gene Expression analysis utilizing 96 genes and 96 samples per nCounter run. Each pack of reagents is sufficient for 1 nCounter runs or 96 assays and requires 12 reaction Master kit. Tier discounting is available for purchase of 8 packs or more (768 samples or more). PlexSet purchases only— does not include Master Kits.

Description

96 Assays

Pack Size

PS-096-GX-96S
(CS0)

SKU

nCounter PlexSet Titration Kit-72 Kit is set up to perform titration for 72-gene format prior to processing samples with PlexSet-72 reagents in 96 sample format. Only one kit is required for the entire project. CodeSet only—does not include Master Kits.

Description

24 Assays

Pack Size

PS-GX-PTK-72
(CS0)

SKU

nCounter PlexSet Titration Kit-12 Kit is set up to perform titration for 12-gene format prior to processing samples with PlexSet-12 reagents in 96 sample format. Only one kit is required for the entire project. CodeSet only—does not include Master Kits

Description

24 Assays

Pack Size

PS-GX-PTK-12 (CS0)

SKU

nCounter PlexSet-72 Reagent Pack For custom Gene Expression analysis utilizing 72 genes and 96 samples per nCounter run. Each pack of reagents is sufficient for 1 nCounter runs or 96 assays and requires 12 reaction Master kit. Tier discounting is available for purchase of 8 packs or more (768 samples or more). PlexSet purchases only— does not include Master Kits.

Description

96 Assays

Pack Size

PS-072-GX-96S (CS0)

SKU

nCounter PlexSet Titration Kit-24 Kit is set up to perform titration for 24-gene format prior to processing samples with PlexSet-24 reagents in 96 sample format. Only one kit is required for the entire project. CodeSet only—does not include Master Kits.

Description

24 Assays

Pack Size

PS-GX-PTK-24 (CS0)

SKU
SKU
Description
Pack Size
Christian Lytle - New multiplexed nCounter® PlexSet™ Reagents- an alternative to qPCR technology

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