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nCounter® Canine IO Panel

nCounter® Canine IO Panel

Prosigna Score / ROR Score:

The ROR Score has been validated to predict the risk of recurrence of disease in ER+ breast cancer after surgery and treatment with 5 years of endocrine therapy. The ROR score depends upon the biology of the intrinsic subtypes, the proliferation score of the tumor, and the tumor size as shown in the equation below:

ROR = aRLumA + bRLumB + cRHer2 + dRBasal + eP + fT

Each of the R variables in the equation above indicate the Pearson correlation coefficient of the PAM50 expression profile for the tumor compared to each of the prototypical centroids for the intrinsic subtypes shown in the heatmap below. P is the proliferation score, which is the average gene expression profile of genes associated with cell-cycle progression and T is the tumor stage.

Figure: Heatmap of PAM50 genes by subtype. Red is higher expression and green is lower expression

nCounter® Canine IO Panel
  • Covering hundreds of genes involved in the immune response of canines to immuno-oncology therapeutics and designed with experts in canine comparative oncology, the nCounterCanine IO Panel is the only gene expression panel built to study the tumor and immune response to IO treatments in dogs.

  • The panel takes advantage of tumor-specific coverage for the most common canine cancers to enable researchers to share data and access enhanced statistical power, potentially improving clinical trial success by translating findings from the treatment of spontaneous canine cancer to humans.

  • Overlapping content with NanoString’sHuman PanCancer IO 360 and PanCancerImmune Profiling Panels provides a suite of panels for comparative studies

GeoMx DSP COVID-19 Protein and RNA Analysis

  • Rapidly perform high-plex spatial analyses of the host response in FFPE or fresh frozen tissue using the  GeoMx Digital Spatial Profiler (DSP). NanoString’s GeoMx DSP platform enables high-plex protein and RNA experiments in key areas of biology such as molecular response, cellular (immune) response, tissue damage, and drivers of individual susceptibility to severe forms of disease.

  • The GeoMx COVID-19 Immune Response Atlas, a ~1,850-plex RNA assay, enables spatial studies of the SARS-CoV-2 virus and host response. RNA targets include COVID-19 receptors and proteases, pulmonary alveolar type I and II markers, lung biology markers, viral response markers, and SARS-CoV-2 probes. RNA targets are profiled simultaneously using the GeoMx DSP and an Illumina next-generation sequencer (NGS) for readout. Users can run ACD RNAscope™ probes alongside GeoMx RNA probes to identify regions of interest.

  • A five-antibody custom, ready-to-go protein panel, with receptor, protease, and viral markers is available through the GeoMx Technology Access Program or for order through Abcam. This  COVID-19 GeoMx-formatted Antibody  is run with the 20-plex GeoMx Immune Cell Profiling Core (plus controls) with readout on the nCounter Analysis System. Users can add up to six 10-plex modules including the Immune Activation Status, Immune Cell Typing, and/or Cell Death modules to more deeply profile proteins involved in T cell activation and cell death. NanoString scientists can recommend commercially-available markers for lung epithelium, nasal epithelium, immune response markers, and the viral spike protein.

Highly Curated Content

Profile 800 genes across 47 annotated pathways involved in canine immune response to IO treatments

PanCancer Coverage

Tumor specific content for top canine cancers including Melanoma, Osteosarcoma, Lymphoma, Urothelial Carcinoma, and Glioblastoma

Rapid and Streamlined Workflow

Easy to use nCounter system provides data in 24 hours with less than 30 minutes of hands-on time and simple data analysis

Flexible Sample Type

Cultured cells/cell lysates, sorted cells, FFPE-derived RNA, total RNA, fragmented RNA, PBMCs, and whole blood/plasma

Customizable

Add up to 55 unique genes with Panel-Plus and up to 10 custom protein targets

Enables researchers to explore canine immune response to immune-oncology treatments

The eight essential components of stem cell biology

Stemness
Pluripotency
Regulatory Signaling
Epigenetics
Mechano-Signaling
Metabolism
Differentiation Signaling
Lineage Specification

Stem Cell Renewal:

  • Stem Cell Prolifiration

  • Cell Cycle

  • Senescence/Quiescence

  • Autophagy

  • Apoptosis

  • Anti-Apoptosis

  • PSC Pluripotency Markers and Regulators

  • Naive State/Primed State

Regulatory Pathways:​

  • Wnt/B-catenin Pathway

  • Hedgehog Signaling

  • AP-1 Signaling

  • PI3K-AKTmTOR Pathway

  • MAPK Pathway

  • JAK/STAT Pathway

  • Notch Signaling

Epigenetic Mechanism:

  • DNA Methylation

  • Histone Acetylation & Methylation

Mechano-Signaling:

  • Rho/ROCK Signaling

  • integrin/Cadherin Signaling

  • Hippo Pathway

Metabolism:

  • Oxidative Stress Response

  • Hypoxia Response

  • Amino Acid Metabolism

  • Fatty Acid Metabolism

  • Glutamine Metabolism

  • Glucose Metabolism

Differentiation Signaling and Pathways:

  • TGFB Signaling

  • Cytoskeletal Reorganization

  • MET/EMT Signaling

  • HOX Gene Activation

Differentiation Lineage:

  • Endodermal /Ectodermal /Mesodermal Lineage Markers

  • Key Somatic Cell Types

Custom & Clinical Products

Assay Type
Product
Maximum Targets
Daily Sample Throughput
Analytes Offered
Common Applications

Custom codeSets

Elements TagSets

PlexSet Reagents

Self-assembled, interchangeable probes, optimized for smaller validation projects with maximum flexibility

Self-assembled, interchangeable probes for high-throughput, sample multiplexing projects

User-designed, turn-key solution that comes ready-to-use

216

96

800

24-96

24-96

192-1152

mRNA, CNV, Fusions

mRNA, Fusions

mRNA, miRNA, CNV, Fusions

Cell line drug screen, biomarker development, infectious disease

Gene Signature Development

Model Organism, Microbes, Agriculture

nCounter Canine IO Panel + Panel Standard: Includes 780 genes; 20 internal reference/housekeeping genes for data normalization. Panel standard includes a pool of synthetic oligonucleotides that correspond to the target sequences in the panel.

Description

12 Reactions

Pack Size

XT-CSPS-CIO-12

SKU
Get Quote

nCounter IO Canine Primer Pool: Primer pools for Low Input RNA profiling of 780 canine genes + 20 internal reference controls. Master Kit, RNA Low Input Kit & Panel CodeSet Required

Description

12 Reactions

Pack Size

LOW-CIO-12

SKU
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nCounter Master Kit:

Reagents, cartridges, and consumables necessary for sample processing on nCounter MAX and FLEX Systems

Description

12 Reactions

Pack Size

NAA-AKIT-012

SKU
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nCounter Sprint Cartridge:

Sample Cartridge for nCounter SPRINT System

Description

12 Reactions

Pack Size

SPRINT-CAR-1.0

SKU
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nCounter SPRINT Reagent Pack containing Reagents A, B, C, and Hybridization Buffer

Description

192 Reactions

Pack Size

SPRINT-REAG-KIT

SKU
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Low Input RNA Reagent Kit: 48rxn kit for profiling from low sample input amounts

Description

48 Reactions

Pack Size

LOW-RNA-48

SKU
Get Quote
SKU
Description
Pack Size

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Please contact us for more info at techsupport@bioinnovations.in

AUTOIMMUNE DISEASE AND CHRONIC INFLAMMATORY DISEASE COVERAGE
Condition
Description

Chronic inflammatory disorder that impacts the lining of joints.

Chronic inflammatory of the digestive tract.

Autoimmune disease is where the immune system mistakenly attacks the insulin-producing cells of the pancreas

Demyelinating disease that disrupts communication with the nervous system.

Immune-mediated disease that causes skin cells to rapidly build up on the surface of the skin

Certain treatments and infections have been reported to interfere with the immune system and induce a series of autoimmune disease or adverse immune-related events.

Chronic inflammatory disease that manifests systematically throughout the body.

Rheumatoid Arthritis

Inflammatory Bowel Disease

Type 1 Diab

Type 1 Diabetes

Multiple Sclerosis

Systematic Lupus Erythematosus

Psoriasis

Induced Adverse Immune Events

Immune Checkpoint Coverage

ADORA2A(A2aR)

CD70

HAVCR2(TIM3)

CD274(PD-L1)

TNFRSF9(4-1BB)

TNFSF9(4-1BBL)

TNFRSF18(GITR)

TNFSF18(GITRL)

VSIR(VISTA)

PDCD1LG2(PD-L2)

TIGIT

TNFRSF4(OX40)

TNFSF4(OX40L)

ICOS

ICOSLG(B7-H2)

LAG3

PDCD1(PD-1)

CD80

CD86

CD276(B7-H3)

CTLA-4

CD27

CD28

CD40

CD40LG

Functional Annotations

Pathways Regulating Glia
(178/180 Hs/Mm Genes)
Inflammation & Peripheral Immune Invasion
(187/188 Hs/Mm Genes)
Cell Stress & Damage Response
(127/134 Hs/Mm Genes)

Angiogenesis

Apoptosis

Autophagy

Cell Cycle

Hypoxia

NO Metabolism and Signaling

Oxidative & Nitrosative Stress

Proteotoxic Stress

Calcium Signaling

Circadian Signaling

Ion Transport

JAK-STAT

Lipid Metabolism

MAPK & PI3K

TGF-Beta Signaling

Wnt Signaling

Antigen Processing & Presentation

Cell Migration

Chemokines

Complement System

Cytokines

Inflammasome

Interferon Signaling

NK-kappaB Signaling

Phagocytosis

T-Cell Signaling

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