Assessment of Tumor Mutational Burden using a Rapid, Amplicon-Based NGS Target Enrichment Strategy.

• The CleanPlex® TMB 500 Panel is a targeted resequencing assay designed to enable rapid and accurate assessment of tumor mutational burden and mutation profile.

• The panel is expertly curated using the latest research findings to target 516 genes associated with major solid tumor types and maintains >96% correlation with whole exome datasets.

• The panel covers 1.64 Mb of genomic content (1.19 Mb exonic) and targets both single nucleotide variants (SNVs) and insertion-deletion mutations (indels).

• Two pools of multiplex PCR primers are utilized to construct target-enriched libraries from either formalin-fixed paraffin-embedded (FFPE) DNA from tumor samples or high-quality gDNA from blood samples. Starting with just 20 ng of DNA (10 ng per primer pool), sequencing-ready libraries can be prepared using a streamlined workflow in just 6 hours, allowing samples to be sequenced on the same day.