Single Cell Sequencing (CTC)
Circulating Tumor Cell Analysis at Single Cell Level
Cancer cells that have shed from the primary tumor are able to invade into surrounding tissues, to intravasate into the bloodstream to become circulating tumor cells (CTCs), at least one part of that cells will be able to generate distant metastases.Cancer cells that have shed from the primary tumor are able to invade into surrounding tissues, to intravasate into the bloodstream to become circulating tumor cells (CTCs), at least one part of that cells will be able to generate distant metastases.
The discovery of CTCs has improved the study of cancer disease as it represents a non invasive biopsy that can be used as prognostic and prediction biomarkers.
Characterization of heterogeneity among CTCs at the single cell level may be useful to better understand the causes and progression of disease and for an accurate selection of molecular prognostic/prediction markers.
Genomic Profiling of Single Circulating Tumor Cell (CTC)
Unlike bulk-cell analysis, single-cell approaches have the advantage of assessing cellular heterogeneity that governs key aspects of tumor biology.
Despite technical challenges, single-cell CTC (scCTC) analyses have so far revealed genomic variations specific to each CTC, including mutations that are not yet present in the Catalogue of Somatic Mutations in Cancer (COSMIC) database
There are a wide range of instruments and methods for capturing, enriching, and enumerating CTCs. Once isolated, CTCs can be studied using next-generation sequencing (NGS) to obtain their genomic profiles.
Targeted sequencing of CTCs using amplicon-based NGS panels is an ideal approach because the workflow is simple to implement and requires little amount of input DNA.
The Most Sensitive Solution For Genomic Profiling Of CTCs
The CleanPlex® OncoZoom® Cancer Hotspot Panel is a multiplex PCR-based targeted sequencing assay designed for rapid detection of somatic mutations across 2,900+ hotspot regions of 65 oncogenes and tumor suppressor genes.
The CleanPlex OncoZoom Cancer Hotspot Panel offers a simple and streamlined workflow. Starting from purified and quantitated DNA, the multiplex PCR-based protocol can be completed in just 3 hours, with 75 minutes of hands-on time, using a three-step, single-tub workflow to minimize sample loss and handling errors. Each step consists of a thermal cycling or incubation condition, followed by “with bead” purification using magnetic beads.