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Sepsis and Resistance Testing

Solutions for quick identification of positive blood cultures and the detection of resistance to them

Sepsis is a life-threatening condition caused by the presence of harmful microorganisms in the bloodstream. It requires prompt diagnosis and appropriate treatment to prevent further complications and reduce mortality rates. One of the key challenges in managing sepsis is the timely identification of the causative pathogens and the determination of their antibiotic resistance profiles. This document discusses the importance of rapid and accurate solutions for achieving fast identification and resistance detection from positive blood cultures, aiding in the effective management of sepsis.

Timely identification of pathogens causing bloodstream infections is crucial for initiating appropriate antimicrobial therapy. Traditional culture-based methods can be time-consuming, taking several days to yield results. Antimicrobial resistance is a growing global concern that complicates the management of sepsis. Effective treatment requires knowledge of the pathogen's susceptibility to specific antibiotics. Resistance detection methods play a vital role in guiding antimicrobial therapy and preventing the spread of resistant strains. Rapid diagnostics, including molecular techniques like polymerase chain reaction (PCR) and next-generation sequencing, can detect resistance genes and mutations efficiently, facilitating targeted therapy.

Bruker has taken further developments to support resistance management by offering a real-time PCR kit to detect the presence of the big 5 carbapenemase genes from a rectal swab, for patients who are at risk of colonization from Carbapenems Producing Enterobacteriaceae (CPE).

Decade, the MALDI Biotyper® has revolutionized microbial identification, offering a fast, easy, robust, reliable, and cost-effective solution. As part of this product evolution, Bruker has focused efforts on finding novel solutions using the MALDI Biotyper® to shorten microbial identification and phenotypic resistance detection of carbapenemase and cephalosporinase, within 60-90 minutes after Positive Blood Culture alert

After subculturing of the PBC, confirmation of resistance mechanisms and quantification of microbial resistance by determination of true MICs is performed using the MICRONAUT broth microdilution (BMD) product portfolio. MICRONAUT provides accurate, complete and true MIC of microorganisms as well as detection of important resistance mechanisms of Ambler class A (ESBL, KPC), class B (MBL), class C (AMP-C) and class D (OXA-48-like) cephalosporinases and/or carbapenems.

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