Dr. Diana Kalinina shall introduce the MALDI Biotyper® sirius platform and elaborate the already available as well as the newly introduced solutions, workflows and accessories that have been made available from Bruker Microbiology & Diagnostics. Additionally, the focus will be around the new MBT Lipid Xtract™ Kit (RUO) kit and related software, for colistin resistance detection.

In addition to proteins, lipids are also abundant molecules in bacteria and could be used to unlock the challenges of microbial identification and antimicrobial susceptibility testing that analysis at proteins level cannot resolve. However, until now, their use in routine clinical diagnostics have not gained a deep interest due to their challenges associated with extractions and analysis. In this presentation, Dr. Gerald Larrouy-Maumus will present the latest advances in the detection of species-specific lipids by the MALDI Biotyper sirius system, their potential use in rapid diagnostics and illustrate the applicability of lipids in routine application by using the MBT Lipid Xtract™ Kit (RUO) and software for rapid detection of colistin-resistant E. coli.

Learning Objectives:

• Identify benefits of the MALDI Biotyper sirius

• Discuss how MALDI-TOF in negative ion mode can be applied to resistance detection and bacterial identification

• Describe colistin resistance and MALDI lipid analysis

For Research Use Only. Not for use in clinical diagnostic procedures. Please contact your local representative for availability in your country.