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Target Screening 3.X Column Kit
Prosigna Score / ROR Score:
The ROR Score has been validated to predict the risk of recurrence of disease in ER+ breast cancer after surgery and treatment with 5 years of endocrine therapy. The ROR score depends upon the biology of the intrinsic subtypes, the proliferation score of the tumor, and the tumor size as shown in the equation below:
ROR = aRLumA + bRLumB + cRHer2 + dRBasal + eP + fT
Each of the R variables in the equation above indicate the Pearson correlation coefficient of the PAM50 expression profile for the tumor compared to each of the prototypical centroids for the intrinsic subtypes shown in the heatmap below. P is the proliferation score, which is the average gene expression profile of genes associated with cell-cycle progression and T is the tumor stage.

Figure: Heatmap of PAM50 genes by subtype. Red is higher expression and green is lower expression

This column is part of the Target Screener solution (3.x and higher), which is used for the analysis of food/water samples for drugs, pesticides or mycotoxins, or equally challenging human/animal urine, saliva, or serum samples for poisons or drugs. It includes three VanGuardpre-columns and one Bruker Intensity Solo 1.8 C18-2 analytical separation column.

GeoMx DSP COVID-19 Protein and RNA Analysis
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Rapidly perform high-plex spatial analyses of the host response in FFPE or fresh frozen tissue using the GeoMx Digital Spatial Profiler (DSP). NanoString’s GeoMx DSP platform enables high-plex protein and RNA experiments in key areas of biology such as molecular response, cellular (immune) response, tissue damage, and drivers of individual susceptibility to severe forms of disease.
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The GeoMx COVID-19 Immune Response Atlas, a ~1,850-plex RNA assay, enables spatial studies of the SARS-CoV-2 virus and host response. RNA targets include COVID-19 receptors and proteases, pulmonary alveolar type I and II markers, lung biology markers, viral response markers, and SARS-CoV-2 probes. RNA targets are profiled simultaneously using the GeoMx DSP and an Illumina next-generation sequencer (NGS) for readout. Users can run ACD RNAscope™ probes alongside GeoMx RNA probes to identify regions of interest.
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A five-antibody custom, ready-to-go protein panel, with receptor, protease, and viral markers is available through the GeoMx Technology Access Program or for order through Abcam. This COVID-19 GeoMx-formatted Antibody is run with the 20-plex GeoMx Immune Cell Profiling Core (plus controls) with readout on the nCounter Analysis System. Users can add up to six 10-plex modules including the Immune Activation Status, Immune Cell Typing, and/or Cell Death modules to more deeply profile proteins involved in T cell activation and cell death. NanoString scientists can recommend commercially-available markers for lung epithelium, nasal epithelium, immune response markers, and the viral spike protein.
The eight essential components of stem cell biology
Stemness
Pluripotency
Regulatory Signaling
Epigenetics
Mechano-Signaling
Metabolism
Differentiation Signaling
Lineage Specification
Stem Cell Renewal:
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Stem Cell Prolifiration
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Cell Cycle
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Senescence/Quiescence
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Autophagy
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Apoptosis
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Anti-Apoptosis
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PSC Pluripotency Markers and Regulators
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Naive State/Primed State
Regulatory Pathways:
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Wnt/B-catenin Pathway
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Hedgehog Signaling
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AP-1 Signaling
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PI3K-AKTmTOR Pathway
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MAPK Pathway
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JAK/STAT Pathway
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Notch Signaling
Epigenetic Mechanism:
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DNA Methylation
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Histone Acetylation & Methylation
Mechano-Signaling:
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Rho/ROCK Signaling
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integrin/Cadherin Signaling
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Hippo Pathway
Metabolism:
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Oxidative Stress Response
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Hypoxia Response
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Amino Acid Metabolism
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Fatty Acid Metabolism
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Glutamine Metabolism
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Glucose Metabolism
Differentiation Signaling and Pathways:
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TGFB Signaling
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Cytoskeletal Reorganization
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MET/EMT Signaling
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HOX Gene Activation
Differentiation Lineage:
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Endodermal /Ectodermal /Mesodermal Lineage Markers
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Key Somatic Cell Types
Custom & Clinical Products
Assay Type
Product
Maximum Targets
Daily Sample Throughput
Analytes Offered
Common Applications
Custom codeSets
Elements TagSets
PlexSet Reagents
Self-assembled, interchangeable probes, optimized for smaller validation projects with maximum flexibility
Self-assembled, interchangeable probes for high-throughput, sample multiplexing projects
User-designed, turn-key solution that comes ready-to-use
216
96
800
24-96
24-96
192-1152
mRNA, CNV, Fusions
mRNA, Fusions
mRNA, miRNA, CNV, Fusions
Cell line drug screen, biomarker development, infectious disease
Gene Signature Development
Model Organism, Microbes, Agriculture
TARGET SCREENING 3.X COLUMN KIT
Description
Complete setup
Pack Size
1855424