nCounter® miRNA Expression Panels
Conventional technologies for small RNA profiling like RNAseq can be costly, onerous, and require complex data analysis. In addition, they require RNA purification and multiple enzymatic steps that can be challenging for biofluid samples like serum and plasma. When developing reliable and robust miRNA signatures for different diseases, you need a robust and reliable platform that works well with multiple sample types.
nCounter miRNA Expression Panels utilize NanoString’s amplification-free technology to do expression profiling by direct quantification of individual RNA molecules. The assay detects miRNAs without the use of reverse transcription or amplification by using molecular barcodes.
Therefore, it is easier and faster to validate miRNA biomarkers as compared to RNASeqor PCR-based platforms and the results are highly reproducible.
Prosigna Score / ROR Score:
The ROR Score has been validated to predict the risk of recurrence of disease in ER+ breast cancer after surgery and treatment with 5 years of endocrine therapy. The ROR score depends upon the biology of the intrinsic subtypes, the proliferation score of the tumor, and the tumor size as shown in the equation below:
ROR = aRLumA + bRLumB + cRHer2 + dRBasal + eP + fT
Each of the R variables in the equation above indicate the Pearson correlation coefficient of the PAM50 expression profile for the tumor compared to each of the prototypical centroids for the intrinsic subtypes shown in the heatmap below. P is the proliferation score, which is the average gene expression profile of genes associated with cell-cycle progression and T is the tumor stage.
Figure: Heatmap of PAM50 genes by subtype. Red is higher expression and green is lower expression
GeoMx DSP COVID-19 Protein and RNA Analysis
Rapidly perform high-plex spatial analyses of the host response in FFPE or fresh frozen tissue using the GeoMx Digital Spatial Profiler (DSP). NanoString’s GeoMx DSP platform enables high-plex protein and RNA experiments in key areas of biology such as molecular response, cellular (immune) response, tissue damage, and drivers of individual susceptibility to severe forms of disease.
The GeoMx COVID-19 Immune Response Atlas, a ~1,850-plex RNA assay, enables spatial studies of the SARS-CoV-2 virus and host response. RNA targets include COVID-19 receptors and proteases, pulmonary alveolar type I and II markers, lung biology markers, viral response markers, and SARS-CoV-2 probes. RNA targets are profiled simultaneously using the GeoMx DSP and an Illumina next-generation sequencer (NGS) for readout. Users can run ACD RNAscope™ probes alongside GeoMx RNA probes to identify regions of interest.
A five-antibody custom, ready-to-go protein panel, with receptor, protease, and viral markers is available through the GeoMx Technology Access Program or for order through Abcam. This COVID-19 GeoMx-formatted Antibody is run with the 20-plex GeoMx Immune Cell Profiling Core (plus controls) with readout on the nCounter Analysis System. Users can add up to six 10-plex modules including the Immune Activation Status, Immune Cell Typing, and/or Cell Death modules to more deeply profile proteins involved in T cell activation and cell death. NanoString scientists can recommend commercially-available markers for lung epithelium, nasal epithelium, immune response markers, and the viral spike protein.
AUTOIMMUNE DISEASE AND CHRONIC INFLAMMATORY DISEASE COVERAGE
Chronic inflammatory disorder that impacts the lining of joints.
Chronic inflammatory of the digestive tract.
Autoimmune disease is where the immune system mistakenly attacks the insulin-producing cells of the pancreas
Demyelinating disease that disrupts communication with the nervous system.
Immune-mediated disease that causes skin cells to rapidly build up on the surface of the skin
Certain treatments and infections have been reported to interfere with the immune system and induce a series of autoimmune disease or adverse immune-related events.
Chronic inflammatory disease that manifests systematically throughout the body.
Inflammatory Bowel Disease
Type 1 Diab
Type 1 Diabetes
Systematic Lupus Erythematosus
Induced Adverse Immune Events
Immune Checkpoint Coverage
Pathways Regulating Glia
(178/180 Hs/Mm Genes)
Inflammation & Peripheral Immune Invasion
(187/188 Hs/Mm Genes)
Cell Stress & Damage Response
(127/134 Hs/Mm Genes)
NO Metabolism and Signaling
Oxidative & Nitrosative Stress
MAPK & PI3K
Antigen Processing & Presentation
Reliably detect and quantitate the most biologically relevant human, mouse, or rat miRNAs directly from FFPE, blood, or biofluids
No Library Preparation
Skip laborious library prep and process your samples with less than one hour of hands-on time
Experience unparalleled reproducibility and specificity with a dynamic range of five logs
Publication Ready Data
Receive publication-ready figures within 24 hours with robust, off-the-shelf data analysis solutions
Provide a sensitive, reproducible, and highly multiplexed method for detecting miRNAs in total RNA across all biological levels of expression.
The eight essential components of stem cell biology
Stem Cell Renewal:
Stem Cell Prolifiration
PSC Pluripotency Markers and Regulators
Naive State/Primed State
Histone Acetylation & Methylation
Oxidative Stress Response
Amino Acid Metabolism
Fatty Acid Metabolism
Differentiation Signaling and Pathways:
HOX Gene Activation
Endodermal /Ectodermal /Mesodermal Lineage Markers
Key Somatic Cell Types
Custom & Clinical Products
Daily Sample Throughput
Self-assembled, interchangeable probes, optimized for smaller validation projects with maximum flexibility
Self-assembled, interchangeable probes for high-throughput, sample multiplexing projects
User-designed, turn-key solution that comes ready-to-use
mRNA, CNV, Fusions
mRNA, miRNA, CNV, Fusions
Cell line drug screen, biomarker development, infectious disease
Gene Signature Development
Model Organism, Microbes, Agriculture
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nCounter miRNA Sample Preparation
nCounter Mouse v1.5 miRNA Expression Assay Kit:
577 miRNAs plus 33 murine associated viral miRNA, 4 miRNA probes, and 23 internal reference controls
nCounter Rat v1.5 miRNA Expression Assay Kit:
423 miRNAs, 4 mRNA and 25 internal reference controls
nCounter a la carte Custom miRNA CodeSets:
Custom miRNA for Human, Mouse, or Rat made from 20-50 miRNAs taken from the list of miRNAs found from Human, Mouse, or Rat Assays
nCounter Human v3 miRNA Expression Assay Kit:
827 miRNAs, 5 mRNAs, and 25 internal reference controls
nCounter MiRGE Codesets to Analyze mRNA & miRNA
nCounter Master Kit:
Reagents, cartridges, and consumables necessary for sample processing on nCounter MAX and FLEX Systems
nCounter Sprint Cartridge:
Sample Cartridge for nCounter SPRINT System
nCounter SPRINT Reagent Pack containing Reagents A, B, C, and Hybridization Buffer