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nCounter® Myeloid Innate Immunity Panel

nCounter® Myeloid Innate Immunity Panel

Prosigna Score / ROR Score:

The ROR Score has been validated to predict the risk of recurrence of disease in ER+ breast cancer after surgery and treatment with 5 years of endocrine therapy. The ROR score depends upon the biology of the intrinsic subtypes, the proliferation score of the tumor, and the tumor size as shown in the equation below:

ROR = aRLumA + bRLumB + cRHer2 + dRBasal + eP + fT

Each of the R variables in the equation above indicate the Pearson correlation coefficient of the PAM50 expression profile for the tumor compared to each of the prototypical centroids for the intrinsic subtypes shown in the heatmap below. P is the proliferation score, which is the average gene expression profile of genes associated with cell-cycle progression and T is the tumor stage.

Figure: Heatmap of PAM50 genes by subtype. Red is higher expression and green is lower expression

nCounter® Myeloid Innate Immunity Panel

The nCounter® Myeloid Innate Immunity Panel provides comprehensive coverage of myeloid-derived cells in a targeted gene expression assay. These panels can be used with multiple sample types like peripheral blood mononuclear cells (PBMCs) or formalin-fixed paraffin-embedded (FFPE) tissue sections and provide results in less than 24 hours with minimal hands-on time and data analysis.


The Myeloid Innate Immunity Panel is designed to encompass all aspects of the innate immune response of myeloid-derived cells and can be used for basic and translational research in immuno-oncology, autoimmunity, and infectious disease.


Each human or mouse panel provides an effective means to comprehensively measure, quantitatively and qualitatively, the attributes of the innate immune response by evaluating relative enrichment of genes that mark recruitment, activation, and effector function of selected myeloid subtype.


Use the Myeloid Innate Immunity Panel to study:

  • Mechanisms of immune evasion

  • Damage response, wound healing & tissue repair

  • Immune regulation

  • Disease pathogenesis

  • Treatment response vs. non-response

Innate Immunity Myeloid Cells

Innate Immunity Myeloid Cells

GeoMx DSP COVID-19 Protein and RNA Analysis

  • Rapidly perform high-plex spatial analyses of the host response in FFPE or fresh frozen tissue using the  GeoMx Digital Spatial Profiler (DSP). NanoString’s GeoMx DSP platform enables high-plex protein and RNA experiments in key areas of biology such as molecular response, cellular (immune) response, tissue damage, and drivers of individual susceptibility to severe forms of disease.

  • The GeoMx COVID-19 Immune Response Atlas, a ~1,850-plex RNA assay, enables spatial studies of the SARS-CoV-2 virus and host response. RNA targets include COVID-19 receptors and proteases, pulmonary alveolar type I and II markers, lung biology markers, viral response markers, and SARS-CoV-2 probes. RNA targets are profiled simultaneously using the GeoMx DSP and an Illumina next-generation sequencer (NGS) for readout. Users can run ACD RNAscope™ probes alongside GeoMx RNA probes to identify regions of interest.

  • A five-antibody custom, ready-to-go protein panel, with receptor, protease, and viral markers is available through the GeoMx Technology Access Program or for order through Abcam. This  COVID-19 GeoMx-formatted Antibody  is run with the 20-plex GeoMx Immune Cell Profiling Core (plus controls) with readout on the nCounter Analysis System. Users can add up to six 10-plex modules including the Immune Activation Status, Immune Cell Typing, and/or Cell Death modules to more deeply profile proteins involved in T cell activation and cell death. NanoString scientists can recommend commercially-available markers for lung epithelium, nasal epithelium, immune response markers, and the viral spike protein.

Insights to Gene Profiling

770 genes In 19 different pathways and processes across 7 different myeloid cell types

Flexible Optimization

Optimized for difficult sample types including FFPE, PBMCs, or FACS sorted cells

Depictions of genes

Genes representing all major myeloid cell types and their various functions including: neutrophils, eosinophils, mast cells, dendritic cells, monocytes, and macrophages with 19 functional and pathway annotations.

Comprehensive Panel

Comprehensive panels for either human or mouse experiments.

Custom-made

Customize with Panel-Plus, a custom spike-in product of 55 genes or less that allows for the creation of a panel and experiment uniquely your own

Rapidly analyze Complex immune responses with publication-quality results next day

The eight essential components of stem cell biology

Stemness
Pluripotency
Regulatory Signaling
Epigenetics
Mechano-Signaling
Metabolism
Differentiation Signaling
Lineage Specification

Stem Cell Renewal:

  • Stem Cell Prolifiration

  • Cell Cycle

  • Senescence/Quiescence

  • Autophagy

  • Apoptosis

  • Anti-Apoptosis

  • PSC Pluripotency Markers and Regulators

  • Naive State/Primed State

Regulatory Pathways:​

  • Wnt/B-catenin Pathway

  • Hedgehog Signaling

  • AP-1 Signaling

  • PI3K-AKTmTOR Pathway

  • MAPK Pathway

  • JAK/STAT Pathway

  • Notch Signaling

Epigenetic Mechanism:

  • DNA Methylation

  • Histone Acetylation & Methylation

Mechano-Signaling:

  • Rho/ROCK Signaling

  • integrin/Cadherin Signaling

  • Hippo Pathway

Metabolism:

  • Oxidative Stress Response

  • Hypoxia Response

  • Amino Acid Metabolism

  • Fatty Acid Metabolism

  • Glutamine Metabolism

  • Glucose Metabolism

Differentiation Signaling and Pathways:

  • TGFB Signaling

  • Cytoskeletal Reorganization

  • MET/EMT Signaling

  • HOX Gene Activation

Differentiation Lineage:

  • Endodermal /Ectodermal /Mesodermal Lineage Markers

  • Key Somatic Cell Types

Custom & Clinical Products

Assay Type
Product
Maximum Targets
Daily Sample Throughput
Analytes Offered
Common Applications

Custom codeSets

Elements TagSets

PlexSet Reagents

Self-assembled, interchangeable probes, optimized for smaller validation projects with maximum flexibility

Self-assembled, interchangeable probes for high-throughput, sample multiplexing projects

User-designed, turn-key solution that comes ready-to-use

216

96

800

24-96

24-96

192-1152

mRNA, CNV, Fusions

mRNA, Fusions

mRNA, miRNA, CNV, Fusions

Cell line drug screen, biomarker development, infectious disease

Gene Signature Development

Model Organism, Microbes, Agriculture

nCounter Human Myeloid Innate Immunity Panel V2 Code Set Only Includes 770 genes, including 40 internal reference genes for data normalization.

Description

12 Reactions

Pack Size

XT-CSO-HMII2-12

SKU

nCounter Mouse Myeloid Innate Immunity Panel V2, Includes 754 genes, including 20 internal reference genes for data normalization.

Description

12 Reactions

Pack Size

XT-CSO-MMII2-12

SKU

nCounter Master Kit (Max or FLEX Systems) Reagents and Cartridges, Reagents, cartridges, and consumables necessary for sample processing on nCounter MAX and FLEX Systems.\

Description

12 Reactions

Pack Size

NAA-AKTI-012

SKU

nCounter Sprint Cartridge:

Sample Cartridge for nCounter SPRINT System

Description

12 Reactions

Pack Size

SPRINT-CAR-1.0

SKU

nCounter SPRINT Reagent Pack containing Reagents A, B, C, and Hybridization Buffer

Description

192 Reactions

Pack Size

SPRINT-REAG-KIT

SKU

Low Input RNA Reagent Kit, 48rxn kit for profiling from low sample input amounts

Description

48 Reactions

Pack Size

LOW-RNA-48

SKU

Human Myeloid Innate Immunity Primer Pools, MTE primer pools for Low Input RNA profiling (770 genes) 730 immune related human genes + 40 internal reference controls. Master Kit, RNA Low Input Kit & Panel CodeSet Required

Description

12 Reactions

Pack Size

PP-HMII2-12

SKU

Mouse Myeloid Innate Immunity, MTE primer pools for Low Input RNA profiling (754 genes) 734 immune-related human genes + 20 internal reference controls. Master Kit, RNA Low Input Kit & Panel CodeSet Required

Description

12 Reactions

Pack Size

PP-MMII2-12

SKU
SKU
Description
Pack Size

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