nCounter® Myeloid Innate Immunity Panel
The nCounter® Myeloid Innate Immunity Panel provides comprehensive coverage of myeloid-derived cells in a targeted gene expression assay. These panels can be used with multiple sample types like peripheral blood mononuclear cells (PBMCs) or formalin-fixed paraffin-embedded (FFPE) tissue sections and provide results in less than 24 hours with minimal hands-on time and data analysis.
The Myeloid Innate Immunity Panel is designed to encompass all aspects of the innate immune response of myeloid-derived cells and can be used for basic and translational research in immuno-oncology, autoimmunity, and infectious disease.
Each human or mouse panel provides an effective means to comprehensively measure, quantitatively and qualitatively, the attributes of the innate immune response by evaluating relative enrichment of genes that mark recruitment, activation, and effector function of selected myeloid subtype.
Use the Myeloid Innate Immunity Panel to study:
Mechanisms of immune evasion
Damage response, wound healing & tissue repair
Treatment response vs. non-response
Prosigna Score / ROR Score:
The ROR Score has been validated to predict the risk of recurrence of disease in ER+ breast cancer after surgery and treatment with 5 years of endocrine therapy. The ROR score depends upon the biology of the intrinsic subtypes, the proliferation score of the tumor, and the tumor size as shown in the equation below:
ROR = aRLumA + bRLumB + cRHer2 + dRBasal + eP + fT
Each of the R variables in the equation above indicate the Pearson correlation coefficient of the PAM50 expression profile for the tumor compared to each of the prototypical centroids for the intrinsic subtypes shown in the heatmap below. P is the proliferation score, which is the average gene expression profile of genes associated with cell-cycle progression and T is the tumor stage.
Figure: Heatmap of PAM50 genes by subtype. Red is higher expression and green is lower expression
Innate Immunity Myeloid Cells
GeoMx DSP COVID-19 Protein and RNA Analysis
Rapidly perform high-plex spatial analyses of the host response in FFPE or fresh frozen tissue using the GeoMx Digital Spatial Profiler (DSP). NanoString’s GeoMx DSP platform enables high-plex protein and RNA experiments in key areas of biology such as molecular response, cellular (immune) response, tissue damage, and drivers of individual susceptibility to severe forms of disease.
The GeoMx COVID-19 Immune Response Atlas, a ~1,850-plex RNA assay, enables spatial studies of the SARS-CoV-2 virus and host response. RNA targets include COVID-19 receptors and proteases, pulmonary alveolar type I and II markers, lung biology markers, viral response markers, and SARS-CoV-2 probes. RNA targets are profiled simultaneously using the GeoMx DSP and an Illumina next-generation sequencer (NGS) for readout. Users can run ACD RNAscope™ probes alongside GeoMx RNA probes to identify regions of interest.
A five-antibody custom, ready-to-go protein panel, with receptor, protease, and viral markers is available through the GeoMx Technology Access Program or for order through Abcam. This COVID-19 GeoMx-formatted Antibody is run with the 20-plex GeoMx Immune Cell Profiling Core (plus controls) with readout on the nCounter Analysis System. Users can add up to six 10-plex modules including the Immune Activation Status, Immune Cell Typing, and/or Cell Death modules to more deeply profile proteins involved in T cell activation and cell death. NanoString scientists can recommend commercially-available markers for lung epithelium, nasal epithelium, immune response markers, and the viral spike protein.
AUTOIMMUNE DISEASE AND CHRONIC INFLAMMATORY DISEASE COVERAGE
Chronic inflammatory disorder that impacts the lining of joints.
Chronic inflammatory of the digestive tract.
Autoimmune disease is where the immune system mistakenly attacks the insulin-producing cells of the pancreas
Demyelinating disease that disrupts communication with the nervous system.
Immune-mediated disease that causes skin cells to rapidly build up on the surface of the skin
Certain treatments and infections have been reported to interfere with the immune system and induce a series of autoimmune disease or adverse immune-related events.
Chronic inflammatory disease that manifests systematically throughout the body.
Inflammatory Bowel Disease
Type 1 Diab
Type 1 Diabetes
Systematic Lupus Erythematosus
Induced Adverse Immune Events
Immune Checkpoint Coverage
Pathways Regulating Glia
(178/180 Hs/Mm Genes)
Inflammation & Peripheral Immune Invasion
(187/188 Hs/Mm Genes)
Cell Stress & Damage Response
(127/134 Hs/Mm Genes)
NO Metabolism and Signaling
Oxidative & Nitrosative Stress
MAPK & PI3K
Antigen Processing & Presentation
Insights to Gene Profiling
770 genes In 19 different pathways and processes across 7 different myeloid cell types
Optimized for difficult sample types including FFPE, PBMCs, or FACS sorted cells
Depictions of genes
Genes representing all major myeloid cell types and their various functions including: neutrophils, eosinophils, mast cells, dendritic cells, monocytes, and macrophages with 19 functional and pathway annotations.
Comprehensive panels for either human or mouse experiments.
Customize with Panel-Plus, a custom spike-in product of 55 genes or less that allows for the creation of a panel and experiment uniquely your own
Rapidly analyze Complex immune responses with publication-quality results next day
The eight essential components of stem cell biology
Stem Cell Renewal:
Stem Cell Prolifiration
PSC Pluripotency Markers and Regulators
Naive State/Primed State
Histone Acetylation & Methylation
Oxidative Stress Response
Amino Acid Metabolism
Fatty Acid Metabolism
Differentiation Signaling and Pathways:
HOX Gene Activation
Endodermal /Ectodermal /Mesodermal Lineage Markers
Key Somatic Cell Types
Custom & Clinical Products
Daily Sample Throughput
Self-assembled, interchangeable probes, optimized for smaller validation projects with maximum flexibility
Self-assembled, interchangeable probes for high-throughput, sample multiplexing projects
User-designed, turn-key solution that comes ready-to-use
mRNA, CNV, Fusions
mRNA, miRNA, CNV, Fusions
Cell line drug screen, biomarker development, infectious disease
Gene Signature Development
Model Organism, Microbes, Agriculture
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nCounter Human Myeloid Innate Immunity Panel V2 Code Set Only Includes 770 genes, including 40 internal reference genes for data normalization.
nCounter Mouse Myeloid Innate Immunity Panel V2, Includes 754 genes, including 20 internal reference genes for data normalization.
nCounter Master Kit (Max or FLEX Systems) Reagents and Cartridges, Reagents, cartridges, and consumables necessary for sample processing on nCounter MAX and FLEX Systems.\
nCounter Sprint Cartridge:
Sample Cartridge for nCounter SPRINT System
nCounter SPRINT Reagent Pack containing Reagents A, B, C, and Hybridization Buffer
Low Input RNA Reagent Kit, 48rxn kit for profiling from low sample input amounts
Human Myeloid Innate Immunity Primer Pools, MTE primer pools for Low Input RNA profiling (770 genes) 730 immune related human genes + 40 internal reference controls. Master Kit, RNA Low Input Kit & Panel CodeSet Required
Mouse Myeloid Innate Immunity, MTE primer pools for Low Input RNA profiling (754 genes) 734 immune-related human genes + 20 internal reference controls. Master Kit, RNA Low Input Kit & Panel CodeSet Required