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nCounter® miRGE Assay

nCounter® miRGE Assay

Prosigna Score / ROR Score:

The ROR Score has been validated to predict the risk of recurrence of disease in ER+ breast cancer after surgery and treatment with 5 years of endocrine therapy. The ROR score depends upon the biology of the intrinsic subtypes, the proliferation score of the tumor, and the tumor size as shown in the equation below:

ROR = aRLumA + bRLumB + cRHer2 + dRBasal + eP + fT

Each of the R variables in the equation above indicate the Pearson correlation coefficient of the PAM50 expression profile for the tumor compared to each of the prototypical centroids for the intrinsic subtypes shown in the heatmap below. P is the proliferation score, which is the average gene expression profile of genes associated with cell-cycle progression and T is the tumor stage.

Figure: Heatmap of PAM50 genes by subtype. Red is higher expression and green is lower expression

nCounter® miRGE Assay
  • Researchers know that simultaneous detection of subsets of mRNA and miRNA in total RNA for biomarker discovery can be tedious and time consuming.

  • The nCounter miRGE assay is uniquely designed to provide a single-tube, sensitive, reproducible, and highly multiplexed method to detect multiple mRNAs and miRNAs simultaneously.

  • After processing with an nCounter miRNA Sample Preparation Kit, mRNA and tagged miRNAs are detected in total RNA without the use of reverse transcription or amplification using nCounter Reporter Probes.

GeoMx DSP COVID-19 Protein and RNA Analysis

  • Rapidly perform high-plex spatial analyses of the host response in FFPE or fresh frozen tissue using the  GeoMx Digital Spatial Profiler (DSP). NanoString’s GeoMx DSP platform enables high-plex protein and RNA experiments in key areas of biology such as molecular response, cellular (immune) response, tissue damage, and drivers of individual susceptibility to severe forms of disease.

  • The GeoMx COVID-19 Immune Response Atlas, a ~1,850-plex RNA assay, enables spatial studies of the SARS-CoV-2 virus and host response. RNA targets include COVID-19 receptors and proteases, pulmonary alveolar type I and II markers, lung biology markers, viral response markers, and SARS-CoV-2 probes. RNA targets are profiled simultaneously using the GeoMx DSP and an Illumina next-generation sequencer (NGS) for readout. Users can run ACD RNAscope™ probes alongside GeoMx RNA probes to identify regions of interest.

  • A five-antibody custom, ready-to-go protein panel, with receptor, protease, and viral markers is available through the GeoMx Technology Access Program or for order through Abcam. This  COVID-19 GeoMx-formatted Antibody  is run with the 20-plex GeoMx Immune Cell Profiling Core (plus controls) with readout on the nCounter Analysis System. Users can add up to six 10-plex modules including the Immune Activation Status, Immune Cell Typing, and/or Cell Death modules to more deeply profile proteins involved in T cell activation and cell death. NanoString scientists can recommend commercially-available markers for lung epithelium, nasal epithelium, immune response markers, and the viral spike protein.

Target Selection

Choose 5–30 miRNA targets from our pre-defined Human, Mouse, or Rat miRNA assays and 100-200 custom Human, Mouse, or Rat mRNA targets for simultaneous analysis

Flexibility

Compatible with total RNA from FFPE, tissue, and cells

Error-free Outcome

No reverse transcription, no amplification, and fewer pipetting steps means less chance for errors and bias in results

Provide a single-tube, ultrasensitive, reproducible, and highly multiplexed method for detecting subsets of both mRNAs and miRNAs in total RNA across all biological levels of expression

The eight essential components of stem cell biology

Stemness
Pluripotency
Regulatory Signaling
Epigenetics
Mechano-Signaling
Metabolism
Differentiation Signaling
Lineage Specification

Stem Cell Renewal:

  • Stem Cell Prolifiration

  • Cell Cycle

  • Senescence/Quiescence

  • Autophagy

  • Apoptosis

  • Anti-Apoptosis

  • PSC Pluripotency Markers and Regulators

  • Naive State/Primed State

Regulatory Pathways:​

  • Wnt/B-catenin Pathway

  • Hedgehog Signaling

  • AP-1 Signaling

  • PI3K-AKTmTOR Pathway

  • MAPK Pathway

  • JAK/STAT Pathway

  • Notch Signaling

Epigenetic Mechanism:

  • DNA Methylation

  • Histone Acetylation & Methylation

Mechano-Signaling:

  • Rho/ROCK Signaling

  • integrin/Cadherin Signaling

  • Hippo Pathway

Metabolism:

  • Oxidative Stress Response

  • Hypoxia Response

  • Amino Acid Metabolism

  • Fatty Acid Metabolism

  • Glutamine Metabolism

  • Glucose Metabolism

Differentiation Signaling and Pathways:

  • TGFB Signaling

  • Cytoskeletal Reorganization

  • MET/EMT Signaling

  • HOX Gene Activation

Differentiation Lineage:

  • Endodermal /Ectodermal /Mesodermal Lineage Markers

  • Key Somatic Cell Types

Custom & Clinical Products

Assay Type
Product
Maximum Targets
Daily Sample Throughput
Analytes Offered
Common Applications

Custom codeSets

Elements TagSets

PlexSet Reagents

Self-assembled, interchangeable probes, optimized for smaller validation projects with maximum flexibility

Self-assembled, interchangeable probes for high-throughput, sample multiplexing projects

User-designed, turn-key solution that comes ready-to-use

216

96

800

24-96

24-96

192-1152

mRNA, CNV, Fusions

mRNA, Fusions

mRNA, miRNA, CNV, Fusions

Cell line drug screen, biomarker development, infectious disease

Gene Signature Development

Model Organism, Microbes, Agriculture

miRGE Assays, Custom assay that simultaneously profiles multiple miRNAs and mRNAs. Base price is for the minimum configuration of 5 miRNAs and 100 mRNAs. Additional miRNA and mRNA targets can be added and incrementally add on to the base price.

Description

48 Assays

Pack Size

MIR-P1CC-48

SKU

miRGE Assays, Custom assay that simultaneously profiles multiple miRNAs and mRNAs. Base price is for the minimum configuration of 5 miRNAs and 100 mRNAs. Additional miRNA and mRNA targets can be added and incrementally add on to the base price.

Description

96 Assays

Pack Size

MIR-P1CC-96

SKU

miRGE Assays, Custom assay that simultaneously profiles multiple miRNAs and mRNAs. Base price is for the minimum configuration of 5 miRNAs and 100 mRNAs. Additional miRNA and mRNA targets can be added and incrementally add on to the base price.

Description

192 Assays

Pack Size

MIR-P1CC-192

SKU

miRGE Assays, Custom assay that simultaneously profiles multiple miRNAs and mRNAs. Base price is for the minimum configuration of 5 miRNAs and 100 mRNAs. Additional miRNA and mRNA targets can be added and incrementally add on to the base price.

Description

384 Assays

Pack Size

MIR-P1CC-384

SKU

miRGE Assays, Custom assay that simultaneously profiles multiple miRNAs and mRNAs. Base price is for the minimum configuration of 5 miRNAs and 100 mRNAs. Additional miRNA and mRNA targets can be added and incrementally add on to the base price.

Description

576 Assays

Pack Size

MIR-P1CC-576

SKU
SKU
Description
Pack Size
nCounter miRGE Sample Preparation

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