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nCounter® Host Response Panel

nCounter® Host Response Panel

Prosigna Score / ROR Score:

The ROR Score has been validated to predict the risk of recurrence of disease in ER+ breast cancer after surgery and treatment with 5 years of endocrine therapy. The ROR score depends upon the biology of the intrinsic subtypes, the proliferation score of the tumor, and the tumor size as shown in the equation below:

ROR = aRLumA + bRLumB + cRHer2 + dRBasal + eP + fT

Each of the R variables in the equation above indicate the Pearson correlation coefficient of the PAM50 expression profile for the tumor compared to each of the prototypical centroids for the intrinsic subtypes shown in the heatmap below. P is the proliferation score, which is the average gene expression profile of genes associated with cell-cycle progression and T is the tumor stage.

Figure: Heatmap of PAM50 genes by subtype. Red is higher expression and green is lower expression

nCounter® Host Response Panel
  • The nCounter® Host Response Panel helps researchers study the phases and progression of infection across the major components of the human or mouse host response with pathogen-agnostic content optimized for blood but suitable for all sample types.

  • Understanding the complex interplay between a pathogen and the host response is important to developing effective vaccines and therapeutics to fight infectious disease.

  • Set up experiments in minutes and get results in less than 24 hours to rapidly advance knowledge about emerging infectious disease.

  • Using this panel you can also identify and validate biomarkers for disease severity and evaluate the effect of vaccines & therapies

GeoMx DSP COVID-19 Protein and RNA Analysis

  • Rapidly perform high-plex spatial analyses of the host response in FFPE or fresh frozen tissue using the  GeoMx Digital Spatial Profiler (DSP). NanoString’s GeoMx DSP platform enables high-plex protein and RNA experiments in key areas of biology such as molecular response, cellular (immune) response, tissue damage, and drivers of individual susceptibility to severe forms of disease.

  • The GeoMx COVID-19 Immune Response Atlas, a ~1,850-plex RNA assay, enables spatial studies of the SARS-CoV-2 virus and host response. RNA targets include COVID-19 receptors and proteases, pulmonary alveolar type I and II markers, lung biology markers, viral response markers, and SARS-CoV-2 probes. RNA targets are profiled simultaneously using the GeoMx DSP and an Illumina next-generation sequencer (NGS) for readout. Users can run ACD RNAscope™ probes alongside GeoMx RNA probes to identify regions of interest.

  • A five-antibody custom, ready-to-go protein panel, with receptor, protease, and viral markers is available through the GeoMx Technology Access Program or for order through Abcam. This  COVID-19 GeoMx-formatted Antibody  is run with the 20-plex GeoMx Immune Cell Profiling Core (plus controls) with readout on the nCounter Analysis System. Users can add up to six 10-plex modules including the Immune Activation Status, Immune Cell Typing, and/or Cell Death modules to more deeply profile proteins involved in T cell activation and cell death. NanoString scientists can recommend commercially-available markers for lung epithelium, nasal epithelium, immune response markers, and the viral spike protein.

Insights to Gene Profiling

Profile 785 human or mouse genes across 50+ pathways

Study elements of host response in human and mouse

Study the five elements of the host response in human and mouse: (i) Host Susceptibility (ii) Interferon Response (iii) Innate Immune Cell Activation (iv) Adaptive Immune Response (v)Homeostasis

Determine pathogen presence

Detect the presence of a pathogen and evaluate organ-specific tissue damage with a Panel Plus spike-in

Flexible Quantification

Quantify the presence and relative abundance of 14 different immune cell types

Developed for understanding the complexities of the immune response to infectious disease, including COVID-19.

The eight essential components of stem cell biology

Stemness
Pluripotency
Regulatory Signaling
Epigenetics
Mechano-Signaling
Metabolism
Differentiation Signaling
Lineage Specification

Stem Cell Renewal:

  • Stem Cell Prolifiration

  • Cell Cycle

  • Senescence/Quiescence

  • Autophagy

  • Apoptosis

  • Anti-Apoptosis

  • PSC Pluripotency Markers and Regulators

  • Naive State/Primed State

Regulatory Pathways:​

  • Wnt/B-catenin Pathway

  • Hedgehog Signaling

  • AP-1 Signaling

  • PI3K-AKTmTOR Pathway

  • MAPK Pathway

  • JAK/STAT Pathway

  • Notch Signaling

Epigenetic Mechanism:

  • DNA Methylation

  • Histone Acetylation & Methylation

Mechano-Signaling:

  • Rho/ROCK Signaling

  • integrin/Cadherin Signaling

  • Hippo Pathway

Metabolism:

  • Oxidative Stress Response

  • Hypoxia Response

  • Amino Acid Metabolism

  • Fatty Acid Metabolism

  • Glutamine Metabolism

  • Glucose Metabolism

Differentiation Signaling and Pathways:

  • TGFB Signaling

  • Cytoskeletal Reorganization

  • MET/EMT Signaling

  • HOX Gene Activation

Differentiation Lineage:

  • Endodermal /Ectodermal /Mesodermal Lineage Markers

  • Key Somatic Cell Types

Custom & Clinical Products

Assay Type
Product
Maximum Targets
Daily Sample Throughput
Analytes Offered
Common Applications

Custom codeSets

Elements TagSets

PlexSet Reagents

Self-assembled, interchangeable probes, optimized for smaller validation projects with maximum flexibility

Self-assembled, interchangeable probes for high-throughput, sample multiplexing projects

User-designed, turn-key solution that comes ready-to-use

216

96

800

24-96

24-96

192-1152

mRNA, CNV, Fusions

mRNA, Fusions

mRNA, miRNA, CNV, Fusions

Cell line drug screen, biomarker development, infectious disease

Gene Signature Development

Model Organism, Microbes, Agriculture

nCounter Human Host Response Panel includes 785 genes; 12 internal reference genes for data normalization

Description

12 Reactions

Pack Size

XT-HHR-12

SKU
Get Quote

nCounter Mouse Host Response Panel includes 785 genes; 12 internal reference genes for data normalization

Description

12 Reactions

Pack Size

XT-MHR-12

SKU
Get Quote

Coronavirus Panel Plus, 20 Probe Panel Plus. Includes 9 probes targeting the SARS-CoV-2 virus, 1 probe for the human gene encoding the viral receptor ACE2, and 10 additional probes targeting SARS and other human coronaviruses

Description

12 Reactions

Pack Size

CORONAPP-12

SKU
Get Quote

Low RNA Input Kit, Kit for use with all Low RNA Input Primer Pools

Description

48 Reactions

Pack Size

LOW-RNA-48

SKU
Get Quote

Primer pools for the Host Response Panel (for use with the Low RNA Input Kit)

Description

12 Reactions

Pack Size

LOW-HHR-12

SKU
Get Quote

nCounter Master Kit:

Reagents, cartridges, and consumables necessary for sample processing on nCounter MAX and FLEX Systems

Description

12 Reactions

Pack Size

NAA-AKIT-012

SKU
Get Quote

nCounter Sprint Cartridge:

Sample Cartridge for nCounter SPRINT System

Description

12 Reactions

Pack Size

SPRINT-CAR-1.0

SKU
Get Quote

nCounter SPRINT Reagent Pack containing Reagents A, B, C, and Hybridization Buffer

Description

192 Reactions

Pack Size

SPRINT-REAG-KIT

SKU
Get Quote
SKU
Description
Pack Size

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Please contact us for more info at techsupport@bioinnovations.in

AUTOIMMUNE DISEASE AND CHRONIC INFLAMMATORY DISEASE COVERAGE
Condition
Description

Chronic inflammatory disorder that impacts the lining of joints.

Chronic inflammatory of the digestive tract.

Autoimmune disease is where the immune system mistakenly attacks the insulin-producing cells of the pancreas

Demyelinating disease that disrupts communication with the nervous system.

Immune-mediated disease that causes skin cells to rapidly build up on the surface of the skin

Certain treatments and infections have been reported to interfere with the immune system and induce a series of autoimmune disease or adverse immune-related events.

Chronic inflammatory disease that manifests systematically throughout the body.

Rheumatoid Arthritis

Inflammatory Bowel Disease

Type 1 Diab

Type 1 Diabetes

Multiple Sclerosis

Systematic Lupus Erythematosus

Psoriasis

Induced Adverse Immune Events

Immune Checkpoint Coverage

ADORA2A(A2aR)

CD70

HAVCR2(TIM3)

CD274(PD-L1)

TNFRSF9(4-1BB)

TNFSF9(4-1BBL)

TNFRSF18(GITR)

TNFSF18(GITRL)

VSIR(VISTA)

PDCD1LG2(PD-L2)

TIGIT

TNFRSF4(OX40)

TNFSF4(OX40L)

ICOS

ICOSLG(B7-H2)

LAG3

PDCD1(PD-1)

CD80

CD86

CD276(B7-H3)

CTLA-4

CD27

CD28

CD40

CD40LG

Functional Annotations

Pathways Regulating Glia
(178/180 Hs/Mm Genes)
Inflammation & Peripheral Immune Invasion
(187/188 Hs/Mm Genes)
Cell Stress & Damage Response
(127/134 Hs/Mm Genes)

Angiogenesis

Apoptosis

Autophagy

Cell Cycle

Hypoxia

NO Metabolism and Signaling

Oxidative & Nitrosative Stress

Proteotoxic Stress

Calcium Signaling

Circadian Signaling

Ion Transport

JAK-STAT

Lipid Metabolism

MAPK & PI3K

TGF-Beta Signaling

Wnt Signaling

Antigen Processing & Presentation

Cell Migration

Chemokines

Complement System

Cytokines

Inflammasome

Interferon Signaling

NK-kappaB Signaling

Phagocytosis

T-Cell Signaling

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