Your content has been submitted
GeoMx Mouse Whole Transcriptome Atlas (WTA)
Prosigna Score / ROR Score:
The ROR Score has been validated to predict the risk of recurrence of disease in ER+ breast cancer after surgery and treatment with 5 years of endocrine therapy. The ROR score depends upon the biology of the intrinsic subtypes, the proliferation score of the tumor, and the tumor size as shown in the equation below:
ROR = aRLumA + bRLumB + cRHer2 + dRBasal + eP + fT
Each of the R variables in the equation above indicate the Pearson correlation coefficient of the PAM50 expression profile for the tumor compared to each of the prototypical centroids for the intrinsic subtypes shown in the heatmap below. P is the proliferation score, which is the average gene expression profile of genes associated with cell-cycle progression and T is the tumor stage.
Figure: Heatmap of PAM50 genes by subtype. Red is higher expression and green is lower expression
Full Transcriptome Spatial RNA Analysis
Mouse models serve a critical role in understanding developmental biology, disease onset, progression, and treatment. Tissue heterogeneity, however, confounds the results from many mouse transcriptome studies based on bulk or single-cell RNA-seq data. Using the GeoMx Mouse Whole Transcriptome Assay, one can apply spatial transcriptomics to reveal the tissue architecture and underlying function in genetically modified mouse models.
GeoMx DSP COVID-19 Protein and RNA Analysis
Rapidly perform high-plex spatial analyses of the host response in FFPE or fresh frozen tissue using the GeoMx Digital Spatial Profiler (DSP). NanoString’s GeoMx DSP platform enables high-plex protein and RNA experiments in key areas of biology such as molecular response, cellular (immune) response, tissue damage, and drivers of individual susceptibility to severe forms of disease.
The GeoMx COVID-19 Immune Response Atlas, a ~1,850-plex RNA assay, enables spatial studies of the SARS-CoV-2 virus and host response. RNA targets include COVID-19 receptors and proteases, pulmonary alveolar type I and II markers, lung biology markers, viral response markers, and SARS-CoV-2 probes. RNA targets are profiled simultaneously using the GeoMx DSP and an Illumina next-generation sequencer (NGS) for readout. Users can run ACD RNAscope™ probes alongside GeoMx RNA probes to identify regions of interest.
A five-antibody custom, ready-to-go protein panel, with receptor, protease, and viral markers is available through the GeoMx Technology Access Program or for order through Abcam. This COVID-19 GeoMx-formatted Antibody is run with the 20-plex GeoMx Immune Cell Profiling Core (plus controls) with readout on the nCounter Analysis System. Users can add up to six 10-plex modules including the Immune Activation Status, Immune Cell Typing, and/or Cell Death modules to more deeply profile proteins involved in T cell activation and cell death. NanoString scientists can recommend commercially-available markers for lung epithelium, nasal epithelium, immune response markers, and the viral spike protein.
Robust & Reproducible
Robust, reproducible results across sample types including FFPE, Frozen Fresh, Fixed Frozen, and TMAs
Whole Transcriptome coverage across all mouse strains
Superior sensitivity detects more genes per sample and quantitates low, medium and high expressed genes
Proven techniques without any specialized mounting or optimization
Spatially Resolved Mouse Transcriptome
The eight essential components of stem cell biology
Stem Cell Renewal:
Stem Cell Prolifiration
PSC Pluripotency Markers and Regulators
Naive State/Primed State
Histone Acetylation & Methylation
Oxidative Stress Response
Amino Acid Metabolism
Fatty Acid Metabolism
Differentiation Signaling and Pathways:
HOX Gene Activation
Endodermal /Ectodermal /Mesodermal Lineage Markers
Key Somatic Cell Types
Custom & Clinical Products
Daily Sample Throughput
Self-assembled, interchangeable probes, optimized for smaller validation projects with maximum flexibility
Self-assembled, interchangeable probes for high-throughput, sample multiplexing projects
User-designed, turn-key solution that comes ready-to-use
mRNA, CNV, Fusions
mRNA, miRNA, CNV, Fusions
Cell line drug screen, biomarker development, infectious disease
Gene Signature Development
Model Organism, Microbes, Agriculture
GeoMx Mouse Whole Transcriptome Atlas Mouse RNA for Illumina Systems: RNA Probe set that targets 21,000+ transcripts for mouse protein coding genes plus ERCC negative controls to profile the whole transcriptome. Excludes uninformative high expressing targets such as ribosomal subunits. Includes RNA probes designed for Illumina NGS readout with the Seq Code library prep.
GeoMx Human Whole Transcriptome Atlas Human RNA for Illumina Systems: RNA Probe set that targets 18,000+ transcripts for human protein coding genes plus ERCC negative controls to profile the whole transcriptome. Excludes uninformative high expressing targets such as ribosomal subunits. Includes RNA probes designed for Illumina NGS readout with the Seq Code library prep.
GeoMx Solid Tumor TME Morphology Kit Human RNA Compatible: Morphology kit for visualization of human solid tumors and the tumor microenvironment. For use with RNA assays. Includes fluorescent antibodies against Pan-CK, CD45, and a nuclear stain.
GeoMx Melanoma TME Morphology Kit Human RNA Compatible: Morphology kit for visualization of human melanoma and the tumor microenvironment. For use with RNA assays. Includes fluorescent antibodies against S100B/Pmel17, CD45, and a nuclear stain.